Fluorescence Polarization Assay

Figure 12 Resynthesized library hits identified from the high throughput fluorescence-polarization assay along with their Src SH2 binding data.

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Figure 4.7 Summary of the measured potencies for A-FABP and selectivities against H-FABP for analogues of BVT.1960. The numbers are based on measurements using the fluorescence polarization assay. Adapted with permission from van Dongen et al., J. Am. Chem. Soc., 124, 11874. Copyright 2002 American Chemical Society.

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We thank B. M. Tempter and C. A. Royer for advice on fluorescence polarization assays. This work was supported by NIH grant GM44783. BRK was supported by NIH Chemistry - Biology Interface training grant GM08505. 

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Table 4.3-2 Results of the fluorescence polarization assay for the terphenyl-based Bak mimetics.

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