Karyoskeletal protein-enriched fraction preparation

Fig. 2 Transmission electron micrograph of karyoskeletal protein-enriched fraction prepared from Drosophila embryos. The main panel shows a nucleuslike structure, bounded by a peripheral lamina (L) Inset Higher magnification showing tangential section through the periphery of karyoskeletal protein structure as shown in the main panel. Nuclear pore complex remnants can be readily appreciated as small ringlike structures.

Preparation of Karyoskeletal Protein-Enriched Fractions from Drosophila melanogaster Cells and Tissues... 

Preparation of D. melanogaster Karyoskeletal Protein-Enriched Fractions... 

Unless otherwise noted, all procedures should be performed at 4°C or on ice. Nuclear purification is the first step in preparation of karyoskeletal protein-enriched fractions from Drosophila cells and tissues. Two techniques have been used extensively in our laboratory. The first, as detailed previously in Fisher et al. (1982), is particularly useful for preparation of undegraded karyoskeletal proteins from embryos without regard for their biological activity. 

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