Protein affinity

By similar logic, protein affinity Hbraries have been constmcted to identify protein—protein combining sites, as in antibody—antigen interaction (19) and recombinant Hbraries have been made which produce a repertoire of antibodies in E. coli (20). In another case, a potential DNA-based therapeutic strategy has been studied (21). DNAs from a partially randomized Hbrary were selected to bind thrombin in vitro. Oligonucleotides, termed aptamers that bound thrombin shared a conserved sequence 14—17 nucleotides long. 

Welch, W.J. Feramisco, J.R. (1985). Rapid purification of mammalian 70,000 Dalton stress proteins Affinity of the proteins for nucleotides. Mol. Cell. Biol. 5, 1229-1237. 

Protein affinity chromatography can be used for the separation of an individual compound, or a group of structurally similar compounds from crude-reaction mixtures, fermentation broths, or cell lysates by exploiting very specific and well-defined molecular interactions... 

Figure 14 6 Silver-stained SDS-PAGE gel of PatA binding proteins. Lane 1, sample 1 nonspecific proteins captured by the streptavidin-agarose resin Lane 2, sample 2 proteins affinity captured by the presence of B-Pat A Lane 3, sample 3 affinity capture of target proteins was blocked by prior addition of free PatA before incubation with B-PatA. The two arrows point to two proteins specifically detected in sample 2 versus sample 1, which were also lost due to competition in sample 3, with apparent molecular weights of 38 and 48 kDa.

Porath, J., and Olin, B. (1983) Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions. Biochemistry 22, 1621-1630. 

Protegrin derivatives, 18 260 Protegrins, 18 260-261 properties of, 18 261 Protein. See also Proteins extraction of, 26 474 in cereal grains, 26 275-276 Proteinaceous materials, as membrane foulants, 21 664 Protein adsorption, 12 136-137 Protein affinity libraries, 12 516-517 Protein-based chiral phases, 6 89-90 Protein-based microarrays, 16 382 Protein biosynthesis, 20 450... 

Techniques to discover the identity of such protein protein interactions are evolving. One approach involves protein affinity chromatography. Here, the purified protein of interest is immobilized on a solid polymer support and proteins that associate with it are identified by electrophoresis and MALDI. There exist a wide number of modifications to the affinity... 

A Lundqvist, E Brekkan, L Haneskog, Q Yang, J Miyake, P Lundahl. Determination of transmembrane protein affinities for solutes by frontal chromatography. In P Lundahl, A Lundqvist, E Greijer, eds. Quantitative Analysis of Biospecific Interactions. Amsterdam Harwood Academic, 1998, pp 79-93. 

Turton S, Gillard NP, Stephenson FA, McKeman RM. Antibodies against the 5-HT3-A receptor identify a 54 kDa protein affinity-purified from NCB20 cells. Mol Neuropharmacol 1993 3 167-171. 

Table 1 Covalent surface immobilization of proteinic affinity ligands [ 124]...

LATCHINIAN, L KHOURI, H.E., IBRAHIM, R.K. Fast protein affinity chromatography of two flavonoid glucosyltransferases. J. Chromatogr., 1987, 388, 235-242. 

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