Affinity-based proteins

Activity-Based and Affinity-Based Protein Profiling. 87... 

The term activity-based protein profiling implies mechanism-based probe/ target reactivity. Photoaffinity labelling approaches represent a complementary technique to mechanism based APBB probes. The use of these photoreactive affinity-based protein profiling probes in proteomic studies are reviewed by Overkleeft et al. 

A number of affinity-based or chromatography methods have been used to prefractionate protein samples for 2D electrophoresis. For example, proteins of low abundance can be enriched from crude lysates by affinity-... 

Affinity-based CEC separations for the analysis of mannose-binding proteins... 

I. Filipuzzi SpeedScreen, a label-free, affinity-based high-throughput screening technology for the discovery of orphan protein ligands, Chimia 2004, 58, 585-587. 

Proteomics can make a key contribution in the identification of interacting protein partners and create a protein-protein interaction map of the cell (Lamond and Mann, 1997 Neubaer et ah, 1997 Blackstock and Weir, 1999 Link et ah, 1999). This would be of immense value to understanding the biology of the cell and can be further exploited for drug development. The simplest way to study such interaction is to purify the entire multiprotein complex by affinity-based methods. The members of the multiprotein complex can then be identified by mass spectrometry. 

Until recently, irreversible chemistries have not been applied to the systematic discovery of novel ligands. Instead, such methods have proven to be more useful for ligand-directed protein discovery, in which ligands specific to a certain protein or class of proteins are used to identify related proteins from a crude cell extract.14 511 Irreversible site-directed ligand discovery forms a continuum with affinity labeling and activity-based protein profiling, in which a reactive (often substrate-like) molecule binds covalently to a protein of interest.152 531 These techniques are typically used to probe enzyme mechanism or function or to identify a specific protein or class of proteins from a complicated mixture such as... 

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