Protein affinity chromatography molecular interaction

Protein affinity chromatography can be used for the separation of an individual compound, or a group of structurally similar compounds from crude-reaction mixtures, fermentation broths, or cell lysates by exploiting very specific and well-defined molecular interactions... 

Subramanian S (1984) Dye-Ugand affinity chromatography the interaction of Cibacron Blue F3GA with proteins and enzymes. Crit Rev Biochem 16(2) 169-205 Sun Y, Xue JL, Dong XY (1995) ModeUing and analysis of the continuous affinity-recycle extraction process a case of specific elution with low molecular weight competitive inhibitor. J Bioproc Biosyst Eng 13(4) 205-210... 

With the availability of labeled hormones of high specific activity and the application of the principles of affinity chromatography, researchers were able to isolate cellular proteins that bind to plant hormones in vitro. Such proteins have been referred to as receptor proteins, binding proteins, or acceptor proteins. Tacit in the concept of hormone receptor proteins is the stereo-specific interaction of the hormone and the receptor protein (19). The resulting hormone-protein complex participates in growth processes that depend on new or enhanced protein synthesis. Advances in molecular biology and related sciences have enabled many researchers to study the role of receptors in the control of nuclear functions or other activities and to determine the site of primary hormonal action. 

Affinity Chromatography was initially defined as a method based on specific and reversible molecular interactions between biologically active substances. However, the method has extended to non-biological stationary phases, such as metal-chelate complexes. This technique is used for separation and purification of proteins and other biologic materials, such as viruses and cells.47,48 A survey of various stationary phases and affinity interactions is given in figure 8.2. 

Affinity chromatography uses the highest selectivity of the protein ligand interaction. The specificity of this interaction, which is a probabilistic term, can be described by the simple law of mass action therefore the association of two molecular species is described by ... 

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