Protein-ligand binding affinity target proteins

Prion Protein Study of the target protein per se and selection of appropriate binding sites Solid-phase combinatorial chemistry (49 ligands) Affinity chromatography ELISA on beads 20... 

The studies described above revealed that BE values can be reliable predictors of ligand binding affinities for target proteins. The BE value can also serve as an additional descriptor to supplement QSAR models. A recent example demonstrated that the statistical quality of a 3D-QSAR model based on comparative molecular field analysis (CoMFA) improved dramatically—from R2 = 0.65 to R2 = 0.93—by inclusion of calculated BE values [86,87]. 

Aptamer is a globular-shaped oligonucleotide that is identified by the systematic evolution of ligands by exponential enrichment (SELEX) process. It has the similar composition as natural nucleic acid, but the nucleotides of aptamer have 2 -modified sugars to enhance the nuclease resistance [122]. Since its discovery in 1990, aptamer has become a valuable research tool. Aptamers can specifically bind to target protein with high affinity, therefore it can be utilized in the targeted delivery of siRNA [37,123]. For example, prostate-specific membrane... 

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