Affinity chromatography protein-ligand combinations

Another peptide was also described for its ability to interact with immunoglobulins and was used as an affinity chromatography ligand.201 This peptide structure resulted from a combinational peptide library screening and was identified by its ability to make complexes with IgG that were displaced by the addition of protein A. 

Suitable pairs of protein and ligand combination for affinity chromatography are antigen-antibody, hormone-receptor, glycoprotein-lectin or enzyme-substrate/cofactor/ effector (Table 3.6). The affinity of lectins for specific carbohydrate moieties (Lis and Sharon, 1973 Barondes, 1981) has made them particularly useful for the purification of distinct groups of glycans and glycoproteins (Table 3.7). 

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