Proteinic affinity ligands

Shuker S B, P J Hadjuk, R P Meadows and R P Fesik 1996. Discovering High-affinity Ligands fc Proteins SAR by NMR. Science 274 1531-1534. 

Thermodynamically it would be expected that a ligand may not have identical affinity for both receptor conformations. This was an assumption in early formulations of conformational selection. For example, differential affinity for protein conformations was proposed for oxygen binding to hemoglobin [17] and for choline derivatives and nicotinic receptors [18]. Furthermore, assume that these conformations exist in an equilibrium defined by an allosteric constant L (defined as [Ra]/[R-i]) and that a ligand [A] has affinity for both conformations defined by equilibrium association constants Ka and aKa, respectively, for the inactive and active states ... 

Wang R, Fang X, Lu Y, Wang S. The PDBbind database collection of binding affinities for protein-ligand complexes with known three-dimensional structures. JMed Chem 2004 47 2977-80. 

For reasons that are not yet clear, skeletal muscle transverse (T)-tubule membranes contain 50-100-fold more high affinity DHP receptors than any other source yet identified [43,45]. Transverse tubule membranes contain 30-70 pmol/mg protein of DHP receptors that bind [ H]PN 200-100 with a of 0.1-0.2nM. The strategy utilized for the purification of L-type channels was similar to that used for the purification of other high affinity ligand binding proteins, and its success was predicted from the prior use of such an approach for the purification of other ion channels [54,55]. Thus the L-type channels were purified as high affinity DHP receptors, with the anticipation that the purified component(s) would constitute functional Ca channels. 

Shuker SB, Hajduk PJ, Meadows RP, Fesik SW. Discovering high-affinity ligands for proteins - SAR by NMR. Science 1996 274 1531-1534. 

Immobilization of Proteins (Enzymes) and Affinity Ligands Mediated by CDI... 

Matrix Attached protein or affinity ligand, or spacer and affinity ligand Ref. 

TABLE 6-3. Immobilization of enzymes and affinity ligands on matrices from polyamides, proteins, or other polymers/copolymers with CDI. 

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