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Polyuridylate

Polynucleotides. Polynucleotides are potent interferon inducers. A mismatched, double-stranded synthetic polyribonucleotide ampligen and the double-stranded acids, polyadenylic-polyuridylic acid and polyinosinic-polycytidylic acids have been widely studied for cancer therapy(ii). Although these materials elicit excellent activity with murine rodents, therapeutic effects are dramatically decreased within primates. [Pg.11]

The reaction of adenosine 5 -phosphoric imidazolide with adenosine analogues on a polyuridylic acid template is discussed in reference [158]. Another polymerization of oligoadenylates (pA)4 and (pA)6 on a polyuridylic acid template in an aqueous system... [Pg.273]

In either case, polyuridylic acid or polythymidylic acid, the rate of dimer formation will not be that calculated for an assemblage of independent sites, as in a solution of mononucleotide or dinucleotide, given by eq. (11). The sites are not independent but are in a connected sequence along a one-dimensional chain, and the rate of dimer formation will begin to deviate from the rate law of eq. (11) as soon as the location of unreacted sites becomes an important factor, certainly when... [Pg.242]

Polyuridylic acid is a polynucleotide which can be used as a synthetic template RNA in a polypeptide synthesizing system (see Glossary for a discussion of these systems). It directs the incorporation of phenylalanine, and the product with poly U as template is polyphenylalanine. It has been observed that prior irradiation of the poly U template reduces its activity as a template.79,80 The amount of phenylalanine incorporation decreases with increasing dose of ultraviolet light, and some serine begins to become incorporated in the polypeptide chain. [Pg.247]

Thus it is important to consider not just thymine dimer but pyrimidine dimers in general as a photochemical lesion,10 and it is obvious that the careful study of the photochemical products is just beginning. Work of the depth and detail which has been carried out on the dinucleotides, UpU72 and CpC,74 and which is beginning to be done on polyuridylic acid, has not yet really been attempted on DNA or... [Pg.261]

A. S. (1976). Factor-free (non-enzymic) and factor-dependent systems of translation of polyuridylic acid by E. coli ribosomes. J. Mol Biol, 101, 537-52. [Pg.279]

Huang, W. M. and. Tso, P O. P. (1966). Physicochemical basis of recognition process in nucleic acid interactions. I. Interactions of polyuridylic acid and nucleosides. J. Mol. Biol, 16, 523. [Pg.281]

Polydeoxyinosinic-deoxycytidylic acid sodium salt Polydeoxyadenylic-thymidylic acid sodium salt Polydeoxyguanylic-deoxycytidylic acid sodium salt Polydeoxyguanylic-polydeoxycytidylic acid sodium salt Polycytidylic-polyguanylic acid sodium salt Polyadenylic-polyuridylic acid sodium salt 8-Oxo-7,8-dihydroguanine Further oxidation products of 8-oxo-G... [Pg.104]

In 1961 Marshall Nirenberg and Heinrich Matthaei reported the first breakthrough. They incubated synthetic polyuridylate, poly(U), with anE. coli extract, GTP, ATP, and a mixture of the 20 amino acids in 20 different tubes, each tube containing a different radioactively labeled amino acid. Because poly(U) mRNA is made up of many successive UUU triplets, it should promote the synthesis of a polypeptide containing only the amino acid encoded... [Pg.1036]

When polyuridylic acid is photolyzed with radiation in the 250 p region, two types of photochemical reactions take place. One is the reversible formation of dimers between adjacent uracils and the other is a photohydration of the 5-6 double bond of the uracil. The position of the photochemical equilibrium of the first reaction is known to depend upon the wavelength of the radiation used and that of the latter is determined by... [Pg.171]

A consideration of the schematic view of a polyuridylic acid molecule shown below indicates that the formation of dimers in a random and... [Pg.172]

An attempt has been made to apply the Cohen and Reiss theory to dimer and hydrate formation in RNA. The results were inconclusive, probably because of a poor choice of example. Application of the theory to RNA was complicated by the necessity of estimating the distribution of uracil residues on the chain. The results are made still more tentative by the fact that Tanaka ignored the probability of dimer formation between cytosine residues, mixed dimers between cytosine and uracil, and hydrate formation in cytosine as well as the resultant deamination phenomena. A better choice of example would have been polyuridylic acid. [Pg.175]

Table 5.23. 1 3C Chemical Shifts (Sc in ppm) and 13P-13C Coupling Constants (in Hz, in Parentheses) of Polyuridylic Acid and Related Monomers [770] Solvent D20, pD 7.8 0.6, Temperature 37 °C (d) = doublet(s), q = quartet). Table 5.23. 1 3C Chemical Shifts (Sc in ppm) and 13P-13C Coupling Constants (in Hz, in Parentheses) of Polyuridylic Acid and Related Monomers [770] Solvent D20, pD 7.8 0.6, Temperature 37 °C (d) = doublet(s), q = quartet).
C Atom Uridine Uridine-5 - phosphate Uridine-3 - phosphate Uridinc-2 - phosphate Polyuridylic acid... [Pg.413]

Nirenberg and Matthaei reported that polyuridylic acid codes for polyphenylalanine. [Pg.884]

Hildenbrand K, Schulte-Frohlinde D (1989) E.S.R. studies on the mechanism of hydroxyl radical-induced strand breakage of polyuridylic add. Int J Radiat Biol 55 725-738 Hildenbrand K, Schulte-Frohlinde D (1997) Time-resolved EPR studies on the reaction rates of per-oxyl radicals of poly(acrylic acid) and of calf thymus DNA with glutathione. Re-examination of a rate constant for DNA. Int J Radiat Biol 71 377-385 Hildenbrand K, Mirtsch S, Schulte-Frohlinde D (1993) H-NMR studies of y-irradiated polynucleotides and DNA in N20-saturated aqueous solutions. Release of undamaged and modified bases. Radiat Res 134 283-294... [Pg.353]

Lysine-rich proteinoids preferentially interact to form microparticles with polycytidylic acid and polyuridylic acid. On the other hand arginine-rich proteinoids interacts much more readily with polyguanylic acid and polyinosinic acid to form such particles under the same condition57>. Lysine-rich proteinoids had most often shown a preferential precipitation with polyuridylic acid in the experiments for interaction between polynucleotides and the proteinoids in the presence of Mg2+ 56). [Pg.74]

Krakauer, H. (1971). The binding ofMg4-+ ions to polyadenylate, polyuridylate, and their complexes. Biopolymers 10(12), 2459—2490. [Pg.390]

Poly U Sepharose Polyuridylic acid mRNA, reverse transcriptase, interferons Pharmacia... [Pg.31]

Not all large molecules are spherical they can also be long random coils with a certain degree of flexibility along the chain. If the chain is flexible, there are rapid local fluctuations between monomers and the dipole-dipole interactions are effectively averaged. An example of this is polyuridylic acid (poly U), which has a molecular weight of 100,000 daltons but is nonetheless very flexible. The line widths for poly U as a random coil are only a few hertz. If the chain is incorporated into a rigid double or triple helix, these local motions are lost and the resonances are broadened beyond detection (see Section 14.7.2). [Pg.252]

Zimmerman SB (1976) The polyuridylic acid complex with polyamines An X-ray fiber observation. J Mol Biol 101 563 — 569... [Pg.531]

Young PR, Kallenbach NR (1978) Secondary structure in polyuridylic acid. Non-classical hydrogen bonding and the function of the ribose 2 -hydroxyl group. J Mol Biol 126 467-479... [Pg.537]

The inhibitions described above occurred only when the analog and polynucleotide contained complementary bases. These combinations are not the only ones in which the interaction can occur, e.g., affinity methods detect some interaction between the non-complementary poly-9-vinyladenine and polyadenylate Apparently, such complexes are too unstable to affect the enzymatic reactions nevertheless, extensive modification of the analog can increase the stability of the polymer-polynucleotide complex to the point where such a polymer can effectively inhibit the reaction. Thus, omisssion of the amino group from poly-9-vinyladenine leads to poly-9-vinylpurine and the latter polymer inhibits the reverse transcription of polyadenylate and polyuridylate The introduction of a dimethylamino group in place of the amino group of poly-9-vinyladenine abolMies all of its inhibitory effects All these effects can be correlated with the ability of polymers to form complexes with templates. [Pg.8]


See other pages where Polyuridylate is mentioned: [Pg.852]    [Pg.239]    [Pg.852]    [Pg.193]    [Pg.212]    [Pg.242]    [Pg.247]    [Pg.262]    [Pg.175]    [Pg.332]    [Pg.412]    [Pg.285]    [Pg.712]    [Pg.209]    [Pg.353]    [Pg.53]    [Pg.56]    [Pg.247]    [Pg.255]    [Pg.591]    [Pg.296]   


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Polyuridylic acid

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