Deoxycytidylic acid

Polydeoxyinosinic-deoxycytidylic acid sodium salt Polydeoxyadenylic-thymidylic acid sodium salt Polydeoxyguanylic-deoxycytidylic acid sodium salt Polydeoxyguanylic-polydeoxycytidylic acid sodium salt Polycytidylic-polyguanylic acid sodium salt Polyadenylic-polyuridylic acid sodium salt 8-Oxo-7,8-dihydroguanine Further oxidation products of 8-oxo-G... 

Deoxyribose Deoxycytidine Deoxycytidylic acid deoxycytidine monophosphate (dCMP) Deoxycytidine diphosphate (dCDP) Deoxycytidine triphosphate (dCTP)... 

Cytosine, C Cytidylic acid, CMP Deoxycytidylic acid, dCMP... 

Note that the insert and plasmid need to be specially modified to link together. "Sticky ends" must first be attached to the insert and the plasmid. The sticky ends attached to the linear insert consist of stretches of deoxycytidylic acid (CCCCCCC). The sticky ends attached to the linear plasmid consist of stretches of deoxyguanidylic acid (GGGGGGGG). The sticky ends consisting of "CCCCCCC" can hybridize with those consisting of "GGGGGGGG." The result is a closed, circular dsDNA. The sticky ends are attached to the insert and plasmid by terminal transferase. Terminal transferase catalyzes the attachment of nucleotides to one end of a linear DNA molecule. 

Using the same rationale as is used above in the purine section, it can be seen from the results in the Table that an extensive back reaction of the deoxyguanylic acid -(Cl2") radical occurs. The slight radioprotective effect of chloride in acidic solutions of deoxycytidylic acid and deoxyadenylic acid may be caused by the inability of these compounds to react with Cl2". 

Cytosine C Cytidylic acid CMP Deoxycytidylic acid dCMP... 

Hydroxymethyl)cytosine has been found in the DNA of the T2, T4 and T phages of . coli [277, 278]. A glycosyl-substituted 5- hydroxymethyl)-2 -deoxycytidylic acid was isolated from a T2, -f-phage [279]. 

For partial-sequence studies, the DNA molecule is hydrolyzed with acid or with specific deoxyribonucleases, and the oligonucleotides are then separated on Dowex columns. By such methods, one-half of the helix has been shown to contain rows of three to five and sometimes more identical deoxypyrimidine nucleotides. For example, three consecutive deoxycytidylic acids and four to five consecutive thymidylic acid residues have been found in DNA obtained from bacteria, plants, and animals. 

Deoxycytidylic acid -f-5,10 H,—Fu. —> Hydioxymethyldeoxy-cytidylie acid FH. Deoxycylidylate hydroxymethjdase (5id)... 

The more recent finding that the virus deoxycytidylic acid was in fact a deoxyribonucleotide of 5-hydroxymethyl cytosine further supports Weed and Cohen s conclusion (322b). 

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