Polymerase chain reaction exponential amplification

The advent of the polymerase chain reaction (PCR) was a major turning point in the history of analytical techniques for biomolecules. Since the first publication of the method in 1985, the exponential signal amplification power of PCR was mirrored by an almost similar exponential increase of applications [1-4]. Using a simple and effective signal amplification by repetitive cyclic duplication of a template nucleic acid strand, the signal-enhancing... 

Polymerase chain reaction (PCR) is an in vitro method for the exponential amplification of a specific DNA region. 

Polymerase chain reaction (PCR) is an amplification technique that is most frequently used in molecular laboratories. The introduction of PCR has increased dramatically the speed and accuracy of DNA and RNA analysis. The PCR is based on exponential and bidirectional amplification of DNA sequences using a set of oligonucleotide primers. ... 

The polymerase chain reaction (PCR) permits exponential amplification of a specific segment of DNA from just a single initial template DNA molecule if the sequence flanking the DNA region to be amplified is known (see Figure 9-24). 

The principle of in vitro selection is governed by a number of the same principles that apply to the Darwinian theory of evolution, as shown in Figure 2. First, the random sequence DNA is prepared by automated solid-phase synthesis. A mixture of four types of nucleotide is added in a stepwise condensation reaction process. When necessary, this DNA library may be converted to an RNA library by in vitro transcription or to a peptide library by in vitro translation. Second, the prepared DNA, RNA, or peptide library is subjected to affinity selection, and the molecules that bind to a target molecule are selected. Because only a very small part of the library is selected in each selection, the selected fraction is then amplified by a polymerase chain reaction (PCR) or a reverse transcription PCR (RT-PCR) technique. Successive selection and amplification cycles bring about an exponential increase in the abundance of the targeting DNA, RNA, or peptide until it dominates the population. 

Reverse transcription polymerase chain reaction (RT-PCR) is an experimental method for exponential amplification of a defined segment of complementary DNA (cDNA) produced by reverse transcription of RNA. 

Polymerase chain reaction (PCR) is an in vitro DNA replication and amplification technique that revolutionized nucleic acid analysis [36]. It enables small amount of nucleic acid molecules to be exponentially amplified (i.e., to generate enough material for their analysis and sequencing). PCR is a commonly used technique in biomedical, molecular biology, and clinical diagnostics laboratories... 

PCR Lab-on-Chip devices are miniaturized fluidic systems for fast DNA amplification. The polymerase chain reaction (PCR) is a molecular biological technique for addressing and exponentially amplifying a DNA fragment. The PCR process is based on tenperature cycles with an enzymatic amplification step. It works without the use of a living cell. For PCR, a liquid mixture of an analyte... 

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