Molecular biological techniques

The use of plants from extreme environments Wild plants from extreme environments may possess genes and gene combinations which confer stress tolerance. We must realise, however, that many of their characteristics, e.g. leaf pubescence and succulence in drought-resistant plants, are incompatible with the high yield potential required for crop plants. In addition, most of these species contain compounds such as phenolics and mucilages which interfere with conventional molecular biology techniques. 

A major requirement for structural studies is the availability of large quantities of pure, functional protein. Essentially all proteins are present in only very small quantities in native cells or tissues, so molecular biology techniques... 

The expression of many of these molecules has been studied during various stages of differentiation of normal neutrophils and also of corresponding leukemic cells employing molecular biology techniques (eg, measurements of their specific mRNAs). For the majority, cDNAs have been isolated and sequenced, amino acid sequences deduced, genes have been localized to specific chromosomal locations, and exons and intron sequences have been defined. Some important proteinases of neutrophils are listed in Table 52-12. 

Use of molecular biology technique to find novel biocatalyst... 

The first two volumes in the series New Comprehensive Biochemistry appeared in 1981. Volume 1 dealt with membrane structure and Volume 2 with membrane transport. The editors of the last volume (the present editor being one of them) tried to provide an overview of the state of the art of the research in that field. Most of the chapters dealt with kinetic approaches aiming to understand the mechanism of the various types of transport of ions and metabolites across biological membranes. Although these methods have not lost their significance, the development of molecular biological techniques and their application in this field has given to the area of membrane transport such a new dimension that the appearance of a volume in the series New Comprehensive Biochemistry devoted to molecular aspects of membrane proteins is warranted. 

Enzymes are mostly determined by some spectrophotometric methods in clinical chemistry laboratories, but immunochemical and molecular biological techniques are finding their way into routine laboratory procedures. 

Narayanan S. Concepts, principles and applications of selected molecular biology techniques in clinical biochemistry. Adv Clin Chem 1996 32, 1-38. 

Narayanan S. Preanalytical and analytical pitfalls in molecular biology techniques. J Clin Ligand Assay 1997 20,200-5. 

Genomics gives us the tools to search for new therapeutic targets by providing new avenues of research. The application of advanced molecular biology techniques in conjunction with a complete understanding of genomic sequences will... 

Due to the ready accessibility of SH2 domains by molecular biology techniques, numerous experimentally determined 3D structures of SH2 domains derived by X-ray crystallography as well as heteronuclear multidimensional NMR spectroscopy are known today. The current version of the protein structure database, accessible to the scientific community by, e.g., the Internet (http //www.rcsb.org/pdb/) contains around 80 entries of SH2 domain structures and complexes thereof. Today, the SH2 domain structures of Hck [62], Src [63-66], Abl [67], Grb2 [68-71], Syp [72], PLCy [73], Fyn [74], SAP [75], Lck [76,77], the C- and N-terminal SH2 domain ofp85a [78-80], and of the tandem SH2 domains Syk [81,82], ZAP70 [83,84], and SHP-2 [85] are determined. All SH2 domains display a conserved 3D structure as can be expected from multiple sequence alignments (Fig. 4). The common structural fold consists of a central three-stranded antiparallel ft sheet that is occasionally extended by one to three additional short strands (Fig. 5). This central ft sheet forms the spine of the domain which is flanked on both sides by regular a helices [49, 50,60]. 

The molecular nature of the neuronal receptors is now becoming understood with the advent of molecular biological techniques. The molecular structure of the mechanosensitive channels has been established only recently. In principle mechanosensitive channels must be opened by mechanical deformation of the neural membrane in which they are em-... 

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