Enzymatic amplification

Saiki RK, Schare S, Faloona F Muelis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia Science 1985 Dec 20, 23o(4732) i35o-i354-... 

Saiki, R. K., etal. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239,487-491. 

Paabo, S. (1989), Ancient DNA Extraction, characterization, molecular cloning and enzymatic amplification, Proc. Natl. Academy of Sciences of the USA 86, 939-943. 

The method of PCR allows selective amplification from a complex genome by enzymatic amplification in vitro. The double-stranded genomic DNA template is denatured by heating, and the temperature is then decreased to allow oligonucleotide primers to hybridize (anneal) to their complementary sequences on opposite strands of the template. The... 

Mullis, K.B., Faloona, F., Scharf, S., Saiki, R., Horn, G. and Erlich, H. (1986) Specific enzymatic amplification of DNA in vitro the polymerase chain reaction. Cold Spring Harbor Symposium of Quantitative Biology 51, 263—273. 

Zarlenga, D.S., Gasbarre, L.C., Boyd, P., Leighton, E. and Lichtenfels, J.R. (1998) Identification and semi-quantitation of Ostertagia ostertagi eggs by enzymatic amplification ofITS-1 sequences. Veterinary Parasitology 77, 245-257. 

Ruano G, Kidd KK, Stephens JC. Haplotype of multiple polymorphisms resolved by enzymatic amplification of single DNA molecules. Proc Natl Acad Sci USA 1990 87[16] 6296-6300. 

F. Pouthas, C. Gentil, D. Cote, and U. Bockelmann, DNA detection on transistor arrays following mutation-specific enzymatic amplification. Appl. Phys. Lett. 84, 1594-1596 (2004). 

Current methods for DNA detection usually require enzymatic amplification of the target DNA sequence prior to analysis. For example, the PCR technique selectively increases the concentration of the target sequence relative to unrelated sequences. PCR methods, however, introduce ambiguities resulting from contamination by different DNA sequence. Therefore, a definitive method is required for the analysis of a single, original DNA sequence. To achieve this objective, the sensitivity and speed of the chemiluminescent enhancement techniques described in this chapter must be improved. 

Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, et al. 1988. Primer-directed enzymatic amplification of... 

C PCR entails enzymatic amplification of specific DNA, " sequences using two oligonucleotide primers that 1 flank the DNA segment to be amplified (fig. 27.2). The primers must complement opposite strands so that after annealing, their 3 ends in effect face each other (see fig. 21.2b). 

S. Paabo, D. M. Irwin, and A. C. Wilson, DNA damage promotes jumping between templates during enzymatic amplification,... 

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