Phosphotransferase system

FIGURE 10.26 Glucose transport in E. coli is mediated by the PEP-dependent phosphotransferase system. Enzyme I is phosphorylated in the first step by PEP. Successive phosphoryl transfers to HPr and Enzyme III in Steps 2 and 3 are followed by transport and phosphorylation of glucose. Enzyme II is the sugar transport channel. [Pg.312]

In this chapter, we have examined coupled transport systems that rely on ATP hydrolysis, on primary gradients of Na or Ff, and on phosphotransferase systems. Suppose you have just discovered an unusual strain of bacteria that transports rhamnose across its plasma membrane. Suggest experiments that would test whether it was linked to any of these other transport systems. [Pg.325]

Meadow, N. D., Fox, D. K., and Roseman, S., 1990. The bacterial phos-phoenolpyrnvate glycose phosphotransferase system. Annual Review of Biochemistry 59 497-542. [Pg.326]

The Enzymes II (E-IIs) of the phosphoenolpyruvate (P-enolpyruvate)-dependent phosphotransferase system (PTS) are carbohydrate transporters found only in prokaryotes. They not only transport hexoses and hexitols, but also pentitols and disaccharides. The PTS substrates are listed in Table I. The abbreviations used (as superscripts) throughout the text for these substrates are as follows Bgl, jS-gluco-side Cel, cellobiose Fru, fructose Glc, glucose Gut, glucitol Lac, lactose Man, mannose Mtl, mannitol Nag, iV-acetylglucosamine Scr, sucrose Sor, sorbose Xtl, xylitol. [Pg.135]

The bacterial phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase systems (PTS) are characterised by their unique mechanism of group translocation. The transported solute is chemically modified (i.e. phos-phorylated) during the process (for comprehensive reviews see [151,152] and... [Pg.300]

Hardesty, C., Ferran, C. and DiRienzo, J. M. (1991). Plasmid-mediated sucrose metabolism in Escherichia colt characterization of scrY, the structural gene for a phosphoenolpyruvate-dependent sucrose phosphotransferase system outer membrane porin, J. Bacteriol, 173, 449-456. [Pg.325]

Saier, M. H. ed. (2002). The Bacterial Phosphotransferase System. Horizon Scientific Press, Norfolk. [Pg.331]

Siebold, C., Flukiger, K., Beutler, R. and Emi, B. (2001). Carbohydrate transporters of the bacterial phosphoenolpyruvate sugar phosphotransferase system (PTS), FEBS Lett. 504, 104-111. [Pg.331]

Eukaryotic ABC transport system Phosphotransferase system (PTS) Ion-coupled transport system Signal Transduction Two-component system Bacterial chemotaxis MAPK signaling pathway Second messenger signaling pathway Ligand-Receptor Interaction G-protein-coupled receptors Ion-channel-linked receptors Cytokine receptors Molecular Assembly Ribosome assembly Flagellar assembly Enzyme assembly... [Pg.388]

PTS, phosphotransferase system PMF, proton motive force. pjSgal, phospho-/ -galactosidase / gal, /1-galactosidase. [Pg.316]

S phosphoenolpyruvate -h glycerone <1> (<1> overall reaction of phosphotransferase system, involved in glycerol dissimilation of Escherichia coli... [Pg.60]

Assev, S. Rolla, G. Evidence for presence of a xylitol phosphotransferase system in Streptococcus mutans OMZ 176. Acta Pathol. Microbiol. Immunol. Scand. Sect. B, 92, 89-92 (1984)... [Pg.63]

H189E <1> (<1> comparable to wild type enzyme [26] <1> thermodynamics [29] <1> mutant retains ability for phosphorylation [34]) [26, 29, 34] Additional information <1, 3, 6, 12> (<1,3,6> overview on mutants [19] <1> N-terminal domain, thermodynamic properties [25,29] <3,6,12> enzyme deletion mutants, virulence of [31] <6> enzyme deletion mutant, alternative pathways [36] <1> fusion protein of enzyme plus the remaining three subunits of glucose phosphotransferase system and Ala-Pro-rich linker sequences [32]) [19, 25, 29, 31, 32]... [Pg.419]

Chauvin, R Brand, L. Roseman, S. Sugar transport by the bacterial phosphotransferase system. Characterization of the Escherichia coli enzyme I monomer/dimer transition kinetics by fluorescence anisotropy. J. Biol. Chem., 269, 20270-20274 (1994)... [Pg.420]

Kohlbrecher, D. Eisermann, R. Hengstenberg, W. Staphylococcal phos-phoenolpyruvate-dependent phosphotransferase system molecular cloning and nucleotide sequence of the Staphylococcus carnosus ptsi gene and expression and complementation studies of the gene product. J. Bacteriol., 174, 2208-2214 (1992)... [Pg.420]

Waygood, E.B. Enzyme I of the phosphoenolpyruvate sugar phosphotransferase system has two sites of phosphorylation per dimer. Biochemistry, 25, 4085-4090 (1986)... [Pg.420]

Hoving, H. Koning, J.H. Robillard, G.T. Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system role of divalent metals in the dimerization and phosphorylation of enzyme I. Biochemistry, 21, 3128-3136 (1982)... [Pg.420]

Durham, D.R. Phibbs, P.V. Fractionation and characterization of the phos-phoenolpyruvate fructose 1-phosphotransferase system from Pseudomonas aeruginosa. J. Bacteriol., 149, 534-541 (1982)... [Pg.421]

Saier, M.H. Schmidt, M.R. Lin, P. Phosphoryl exchange reaction catalyzed by enzyme I of the bacterial phosphoenolpyruvate sugar phosphotransferase system. Kinetic characterization. J. Biol. Chem., 255, 8579-8584 (1980)... [Pg.421]

Robillard, G.T. Dooijewaard, G. Lolkema, J. Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. Complete purification of enzyme I by hydrophobic interaction chromatography. Biochemistry, 18, 2984-2989 (1979)... [Pg.421]

Jaffor Ullah, A.H. Cirillo, V.P. Mycoplasma phosphoenolpyruvate-depen-dent sugar phosphotransferase system purification and characterization of enzyme I. J. Bacteriol., 131, 988-996 (1977)... [Pg.421]

Postma, P.W. Roseman, S. The bacterial phosphoenolpyruvate sugar phosphotransferase system. Biochim. Biophys. Acta, 457, 213-257 (1976)... [Pg.421]

De Reuse, H. Danchin, A. The ptsH, ptsI, and err genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system a complex operon with several modes of transcription. J. Bacteriol., 170, 3827-3837 (1988)... [Pg.421]

Alice, A.E Perez-Martinez, G. Sanchez-Rivas, C. Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine metabolism in Bacillus sphaericus. Microbiology, 149, 1687-1698 (2003)... [Pg.421]

Chauvin, F. Fomenkov, A. Johnson, C.R. Roseman, S. The N-terminal domain of Escherichia coli enzyme I of the phosphoenolpyruvate/glycose phosphotransferase system molecular cloning and characterization. Proc. Natl. Acad. Sci.USA, 93, 7028-7031 (1996)... [Pg.421]

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