Phenolic compounds biosensors

A derived combined approach uses an amperometric biosensor [57] with a whole-cell (E. coli) sensing part, for industrial application (textile and tannery wastewaters) and detection of phenolic compounds, non-ionic surfactants and benzenesulphonate compounds. As in the previous studies, chemical analysis (SSPE followed by LC-MS) revealed the pollutants responsible for the observed toxicity. 

Gutes, A., Cespedes, F., Alegret, S., and del Valle, M. (2005). Determination of phenolic compounds by a polyphenol oxidase amperometric biosensor and artificial neural network analysis. Biosens. Bioelectron. 20(8), 1668-1673. 

Relative response (%) of the biosensor to different phenolic compounds... 

J. Svitel and S. Miertus, Development of tyrosinase based biosensor and its application for monitoring of bioremediation of phenol and phenolic compounds, Environ. Sci. Technol., 32 (1998) 828-832. 

Enzyme-based biosensors are very suitable for the antioxidant status evaluation, since they show excellent selectivity for biological substances and can directly determine and/or monitor antioxidant compounds in a complex media such as biological or vegetable samples without needing a prior separation step. During the course of the catalytic reaction on the electroactive substrates, the current produced at an applied potential is related to the concentration of a specific biomarker, for which the biosensor is selective. HRP-based biosensors for antioxidant status evaluation have been applied in the detection of superoxide radical [119], nitric oxide [120], glutathione [119, 121], uric acid [122, 123], and phenolic compounds [124—126],... 

Ramos MC, Torrijas MC, Diaz AN (2001) Enhanced chemiluminiscence biosensor for the determination of phenolic compounds and hydrogen peroxide. Sens Actuators 73 71-75... 

The proposed biosensor has been used for nanomolar detection of various phenolic compounds (phenol, p-cresol and phenol). Above results indicates that Fe304 nanoparticles played an important role in immobilizing tyrosinase and enhancing the enzyme catalytic sites... 

Biosensors based on a Clark oxygen electrode, coupled to tyrosinase immobilized by three different methods, were investigated for the determination of phenol in real matrices, such as water of various natural sources, industrial wastes and oil press. The feasibility study included direct use of the biosensors and in situ analysis. An integrated system, incorporating SPE, desorption, fractionation and biosensor detection, was validated for screening phenolic compounds in water. Two types of electrode were tested, solid graphite and CPE incorporating tyrosinase. Correct analyses were found for river water samples spiked with phenol (10 p.gL ), p-cresol (25 p.gL ) and catechol (1 A mul-... 

The enhanced chemiluminescense obtained with the horseradish peroxidase-H202-luminol (139) system was applied to the development of a CLD biosensor for p-iodophenol, coumaric acid (26), 2-naphthol and hydrogen peroxide. The enzyme was immobilized by microencapsulation in a sol-gel matrix. LOD for the phenolic compounds were 0.83 p,M, 15 nM and 48 nM, respectively. A remote version of the enhanced biosensor was designed by directly immobilizing the enzyme on the tip of an optical fiber. This model was used for H2O2 assay. LOD was 52.2 p,M, with RSD 4.7% (w = 4) °. A bioluminescent response was obtained for phenols with pA a > 7 in the presence of a recombinant Escherichia coli strain, DPD2540, containing a fabA luxCDABE fusion this behavior may have analytical applications. 

Trametes versicolor laccase Graphite Biosensors for phenolic compounds [81]... 

Nandakumar R. and Mattiasson B., A microbial biosensor using Pseudomonas putida cells immobilized in expanded bed reactors for the on-line monitoring of phenolic compounds, A aZ. Lett., 32, 2379-2393, 1999. 

Reports on the use of monomeric MPc complexes for the electrocatalysis of phenols are rare, hence the use of some polymeric MPc complexes will be included in this section. Biosensors based on enzymes have been developed for many electrochemical analyses. The use of MPc complexes as part of the enzyme improves the sensitivities of the biosensors. Ozsoz et al. used CoPc monomer dispersed in mushroom tissue electrode as a catalyst for the analysis of phenolic compounds The enzymatic reaction between mushroom and the phenolic compounds was coupled with the catalytic activity of CoPc. The CoPc dispersed electrodes gave shorter response times and lower potentials compared to conventional tissue biosensors . 

Ozsoz, M., A. Erdem, E. Kdinc, and L. Gokgunnec (1996). Mushroom-based cobalt phthalocyanine dispersed amperometric biosensor for the determinination of phenolic compounds. Electroanalysis 8(2), 147-150. 

Cellobiose Dehydrogenase and Peroxidase Biosensors for Determination of Phenolic Compounds... 

Wang, J. (2008) Electrochemical glucose biosensors. Chem. Rev., 108,814-825. Scampicchio, M., Arecchi, A., Lawrence, N.S., and Mannino, S. (2010) Nylon nanofibrous membrane for mediated glucose biosensing. Sens. Actuators, B, 145,394-397. Arecchi, A., Scampicchio, M., Drusch, S., and Mannino, S. (2010) Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds. Anal. Chim. Acta, 659, 133-136. 

Chen X., Cheng G., Dong S. Amperometric tyrosinase biosensor based on a sol-gel-derived titanium oxide-copolymer composite matrix for detection of phenolic compounds. Analyst 2001 126 1728-1132... 

Some of the most important chemicals naturally occurring in grapes and wines are phenolic compounds. The major quality properties of wines, such as flavor, color stability, and antioxidant capacity are strongly related to phenolic compounds. The study of Amarita et al. (2010) presented an alternative method for the rapid analysis of total phe-nolics in wines and juices based on an enzymatic FIA system. The design of the biosensor used the measurement of the oxygen consumed in the reaction catalyzed by the enzyme... 

Moczko, E., et al. Biosensor employing screen-printed PEDOTPSS for sensitive detection of phenolic compounds in water. J. Polym. Sd., Part A Polym. Chem. 50(11), 2286-2292 (2012)... 

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