Peptide motifs

Protein-protein interaction domain that binds to short peptide motif at the C-terminal of target proteins. Particularly important in spatial organization of receptors and ion channels. 

Mrksich, M., M.E. Parks, and P.B. Dervan. Hairpin peptide motif A new class of oligopeptides for sequence-specific recognition in the minor-groove of double-helical DNA. J. Am. Chem. Soc. 1994, 116, 7983-7988. 

Bisoffi, M., Marti, S. and Betschart, B. (1996) Repetitive peptide motifs in the cuticlin of Ascaris suum. Mokcubr and Biochemical Parasitobgy 80, 55-64. 

The neoangiogenic tumor vasculature overexpresses certain integrins and other surface markers, which can also be used for targeting of polyplexes. The RGD peptide motif has been successfully applied for integrin-targeted pDNA [125-128] and siRNA [129, 130] delivery. In many cases, the PEG motif-containing peptide was attached to the polycation via a PEG spacer. For RGD-PEG-PEI/pDNA polyplexes, an optimum grafting with RGD-PEG was required because transfection... 

A specific peptide motif linked to the chemotherapeutic molecule can enhance its efficacy, reduce their toxicity and also improve tumour cell targeting.45,51,52 Therefore, a range of glycoporphyrins 59 and 60 bearing the Arginine-Glycine-Aspartate (RGD motif) moiety were synthesised (Fig. 6). 

Martin-Serrano, j., Zang, T., and Bieniasz, P. D. HlV-1 and Ebola virus encode small peptide motifs that recruit TsglOl to sites of particle assembly to facilitate egress. Nature Med. 2001, 7, 1313-19. 

Igarashi, K., Kaneda, M., Yamaji, A., Saido, T.C., Kikkawa, U., Qno, Y., Inoue, K., and Umeda, M., 1995, A novel phosphatidylserine-binding peptide motif defined by an anti-idiotypic monoclonal antibody. Localization of phosphatidylserine-specific binding sites on protein kinase C and phosphatidylserine decarboxylase. J. Biol. Chem. 270 29075-29078. 

Erard M, Lakhdar-Ghazal F, Amalric F (1990) Repeat peptide motifs which contain beta-turns and modulate DNA condensation in chromatin. Eur J Biochem 191 19—26 Erard MS, Belenguer P, Caizergues-Ferrer M, Pantaloni A, Amalric F (1988) A major nucleolar protein, nucleolin, induces chromatin decondensation by binding to histone HI. Eur J Biochem 175 525—530 Falaschi A (2000) Eukaryotic DNA replication a model for a fixed double replisome. Trends Genet 16 88-92... 

Table 4. Selected examples of short peptide motifs derived from phage display peptide libraries (adapted from [116]) ...

Table 4 lists some examples of proteins that bind short peptide motifs derived from phage display peptide libraries [66,116-121]. Of particular interest is the use of phage display peptide libraries for screening peptides that bind to integrins. Each of the integrins and has a slightly different... 

This review will focus on the use of MCR approaches to cyclic peptides, cyclic peptidomimetics, or cyclic pseudopeptides, including small or medium-sized heterocycles as mimics of peptide motifs and macrocycles with amino acid or peptide moieties. 

Figure 2. Protein interaction domains are used by E3 protein-ubiquitin ligases to recruit substrates for ubiquitination, as well as E2 enzymes. Ubiquitination targets contain specific peptide motifs which bind interaction domains in the E3 protein/complex. These interactions can require phosphorylation on tyrosine (pTyr) or threonine (pThr), or the presence of proline-rich motifs (PPXY). The papilloma vims E6/E6AP complex can recognize substrates that contain PDZ domains, which bind a C-terminal motif in the E6 viral protein. See text for details.

De Ciechi, P.A., C.S. Devine, S.C. Lee, S.C. Howard, P.O. Olins, and M.H. Caparon, Utilization of multiple phage display libraries for the identification of dissimilar peptide motifs that bind to a B7-1 monoclonal antibody. Mol Divers, 1996.1(2) 79-86. 

Kawamura, Y., Takane, T., Stake, M., and Sugimoto, T. (1992). Physiologically active peptide motif in proteins Peptide inhibitors of ACE from the hydrolysates of Antarctic krill muscle protein. Jpn. Agric. Res. Quart. 26, 210-213. 

The selectivity of peptide motifs for certain metals comes from the coordinating contribution from amino acid side chains, the common coordination number of the metal, hardness/softness of the metal ion, ligand field stabilisation effects and the hardness/softness of any coordinating side chains of the amino acid sequence. An example of the influence of side chains and the importance of the position of the side chain comes from the tripeptides Gly-Gly-His, also known as copper binding peptide. The side chain imidazole ring of the His residue has a very efficient nitrogen donor (the imidazole N), which can form a tetradentate chelate ring for coordination as in Scheme 10.3. 

Coincidentally, by aiming the peptide N-terminal amino group in our 3-D ligand-receptor complex toward Glu-347, rather than Asp-256,32 we identified a hydrophobic pocket as a possible component for interaction with Phe-43 in the agonist peptide motif. This hydrophobic cluster involves residues Phe-182, Leu-340, Tyr-337, and Phe-339 (Figure 9). Assignment of the stop and start residues for... 

Rammensee H, Bachmann J, Emmerich NP et al (1999) SYFPEITHI database for MHC ligands and peptide motifs. Immunogenetics 50 213-219... 

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