Pectin citrus fruit juice

Citrus fruit, juice - 382, 615, 788 Citrus pectin liquor - 840, 861 Clarifier liquid - 679 Clay - 615... 

Pectin is a long chain of pectic acid and pectinic acid molecules. Because these acids are sugars, pectin is categorized as a polysaccharide. It is prepared from citrus peels and the remains of apples after they are squeezed for juice. In the plant, pectin is the material that joins the plant cells together. When fungus enzymes break down the pectin in fruit, the fruit gets soft and mushy. 

Pectin is used in foods in two forms, high methoxyl pectin and low methoxyl pectin. High methoxyl pectin is the form normally found in fruit while low methoxyl pectin is a chemically modified pectin. Pectins are acidic polysaccharides that occur in the cell walls of fruit. The commercial source of pectin is either citrus peel or apple pomace. The citrus peel is the residue from the production of citrus juices while apple pomace is the residue of cider production. Thus pectin is a by-product of either cider or fruit juice production. 

Botanically speaking, citrus is a hesperidium, a berry with a leathery aromatic rind and a fleshy interior divided into sections. As shown by the cross section shown in Fig. 6.1, the exo carp or peel consists of an outer layer called the flavedo which contains oil glands and pigments and a white spongy inner layer called the albedo. The fleshy interior or endocarp of the fruit consists of wedge-shaped sections (segments) filled with multiple fluid-filled sacs or vesicles. These juice sacs constitute the edible portion of a citrus fruit. The cytoplasm contents provide the primary source of the citrus juice. The juice consists primarily of water, sugars, pectins, lipids, terpenes, amino acids, phenolics, carotenoids and minerals. 

The only sources of NSP in soft drinks are fruit materials, gums and stabilisers such as sodium carboxymethylcellulose (CMC) and pectins. Of these items, only fruit juices are used in significant quantities. The NSP content of most fruits falls within the range 0.9-3.6% w/w but, as Table 13.4 shows, very little remains in processed juices. Citrus comminutes are conventionally considered to have the same NSP content as the corresponding fruit but if they are known to contain substantial amounts of peel extracts this should be accounted for in calculations. 

Citrus fruits, especially certain of their component parts, constitute one of the richest sources of pectin. On a dry weight basis, as much as 30% of orange fruit albedo may be pectin (8). The rag, comprising the fruit core and segment membranes after juice extraction, is also a rich source. Since pectin is a cell wall component, it follows that comparatively little would be present in juice expressed from fruit. For example, concentrations ranging from 0.01 to 0.13% in orange juice have been reported (15). Much of this would be present as cell wall fragments and particulate material in juice pulp and cloud. 

The quality of extracted citrus juices depends on enzyme reactions that occur not only in the fruit during the development period, but also in the juice during processing. When juice is extracted from citrus fruit, enzymes are released from their normal restraint in the cell. Several of these enzymes catalyze reactions that adversely affect taste and appearance of the juice. Unless the reactions are controlled, the juice products will not meet the standards of quality set up by the USDA Food Safety and Quality Service. The two reactions of commercial importance are the hydrolysis of pectin to pectic acid, which clarifies juice, and the lactonization of limonoic acid A-ring lactone to the bitter compound, limonin. Research efforts to identify and characterize the reactions, to isolate and purify the enzymes, and to develop methods to control the reactions are described in this review. 

Pectin methylesterase Aspergillus sp Apple and red berry juice pressing Citrus fruit peeling... 

Pectin is obtained from unripe apples or citrus fruit. In both cases the residues left after pressing to extract juice are used for pectin production. In the fruit, pectin is responsible for the firm structure. Apples, for example, have a firm texture despite a water content of more than 85%. Pectin is sold almost exclusively in powder form. 

The pulp wash liquid from citrus fruit contains a higher amount of pectin than the single strength juice from the juice extractors. In most cases it must be treated enzymatically to achieve a sufficiently high concentration in the evaporator. In order to aid the concentration it is also commonly practised to centrifuge the liquid prior to evaporation to remove some or most of the insoluble solids from the liquid. 

Nearly 5 million metric tons of citrus peel and pulp waste materials are produced annually In the manufacturing of concentrated citrus juices In Florida. Although these materials are currently processed Into cattle feed, they are rich In polysaccharides and can be considered as a source of dietary fiber. Alcohol-Insoluble solids (AIS) of 3 different fractions of the processing residues discharged from commercial juice manufacturing equipment were analyzed for pectin, easily hydrolyzable polysaccharides, and cellulose. Two varieties of orange and one of grapefruit that are the predominant citrus fruit used in Juice production were included In this study. 

Marmalade is a spreadable preparation made from pulp, slurry, juice, aqueous extracts or peels of citrus fruits and sugars. The product (1 kg) has to contain at least 200 g of citrus fruit (of which 75 g endocarp) and 60% by weight of soluble solids. The addition of fruit pectin and starch syrup are customary. 

Extraction and Identification. Identification of PE as the clearing enzyme in citrus juices progressed rapidly after MacDonnell et al. (17) reported on cation requirement for extraction and solubilization of the enzyme from various portions of the fruit. PE was assayed by the method introduced by Kertesz (18) and modified by Lineweaver and Ballou (19). The method involved measuring the rate at which the methyl ester groups in the pectin molecule are hydrolyzed by titrating the free carboxyl groups with 0.1N NaOH as they are formed. One unit of PE was defined as the amount of enzyme which will hydrolyze 1 meq carboxyl groups per min from a 0.5% solution of pectin in 0.15M NaCl at pH 7.5, 30°C (86°F). McDonnell et al. (17) showed that the enzyme... 

Cloud stability in citrus juices Pectin manufacture from citrus pomace Distillates from fermented fruit pulps Enzymatic maceration of fruits and vegetables Self-clarification of lemon/lime and apple juices Pressing characteristics of citrus pomace Ca2 -firming of fruits and vegetables... 

Versteeg et al. (50) have isolated various multiple forms of pectin esterases in oranges and shown that they differ in affinity to pectins and pectates and in heat stability and therefore may play different roles in cloud loss phenomena. One form (about 5% of the total PE activity) was found to be much more heat stable than the other forms it was also active at low pH (2.5) and at low temperatures. Another form did not cause self-clarification for this enzyme a similar mode of attack was proposed as for fungal pectin esterases which produce low-ester pectins less sensitive to calcium. Multiple forms of pectin esterases are present in the fruits of all of the orange varieties and citrus species tested. The two isoenzymes known to be responsible for cloud loss and gelation in citrus products were found to occur in all of the component parts of the orange fruit (51). In the French cider industry the endogenous PE of apple is used for the self-clarification of apple juice (52). 

The methanol content in citrus juices ranges from 24 to 47 mg/1, its content in ciders is 36-88 mg/1, and in blackcurrant juices reaches 70-176 mg/1. The methanol content of fruit wines is higher usually ranges from 20 to 240 mg/1, but, exceptionally, it may reach even more than 600 mg/1, because a further portion of the pectin is hydrolysed during the fermentation process. The amount of methanol formed in wines depends on many factors. Red wines have about twice as high a methanol content as white wines. Vodka has... 

Citrus juices are not as nutritious as the whole edible portion of the fruits including all but the seeds and the peel, because substances such as fiber, pectin, and bioflavanoids are present mainly in the peel and the membranes which surround the segments of fruit... 

Pectin— This material is extracted from citrus peel and apple pomace (the residue after extraction of juice). It is available in pure form, or in mixtures that contain added sugar and acid and are used to prepare jams and jellies. Although pure citrus pectin has been given orally in the powdered form (1 or 2 tsp [5 or 10 ml per day) to counteract the effects of dietary cholesterol, a more desirable use for it is in low-calorie recipes such as imitation or eggless mayonnaise, tomato aspic, fruit desserts, and pie filling. It is also used in antidiarrheal preparations. 

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