Orange, fruit

De Pee, S. et al.. Orange fruit is more effective than are dark-green, leafy vegetables in increasing serum concentrations of retinol and (3-carotene in school children in Indonesia, Am. J. Clin. Nutr, 68, 1058, 1998. 

Maccarone, E., Maccarrone, A., and Rapisarda, P, Color stabilization of orange fruit juice by tannic acid, Int. J. Food. Sci. TechnoL, 22, 159, 1987. 

Versteeg, C. 1979. Pectinesterases from the orange fruit - Their purification, general characteristic and juice cloud destabilizing properties. Ph.D. thesis. Agricultural Univ., The Netherlands. 

Isolation, characterization and inununo localization of orange fruit acetyl esterase. 

Immuno localization using polyclonal antibodies raised against AE showed that AE was widely distributed in orange fruit but with more intensive immunological detection in the outer part of the peels e.g. albedo and flavedo and in the segments (juice vesicles). The results indicate that AE is located at the site where the major fraction of pectin is deposited. 

The present work reports the purification and characterization of acetyl esterase from orange fruit as well as the in situ localization of the enzyme by immuno histology. 

Contrary to AE from orange peels described by Williamson (2) this AE has a higher molecular mass and pi > 9. AE isolated from mung bean, however, has. similar MW and pi in agreement with the present findings (1). These differences could indicate the presence of several AE isoformes in different varieties of orange fruits. 

Immuno localizations of AE in sections of orange fruits are shown in Fig. 3. The most intensive depositions of acetyl esterase were found in the outermost parts of the peel (exocarp or outermost albedo and the flavedo) and in the segments (juice vesicles), although quite high levels of acetyl esterase were found in most other tissues as well. The acetyl esterase depositions were all intracellular. 

Collect 2 kg of orange fruits randomly and use 1 kg for juice and the other 1 kg for pulp and rind. Prepare the juice by crushing oranges directly with a juicer mixer. 

Rapisarda P, Bellomo SE and Intelisano S. 2001. Storage temperature effects on blood orange fruit quality. 

Yellow or orange fruits, including pumpkins, oranges, and peaches, which primarily contain xanthophyll esters... 

Christensen TMIE, Nielsen JE, Mikkelsen JD. Isolation, characterization and immunolocalization of orange fruit acetyl esterase, in Progress in Biotechnology 14 Pectins and Pectinases (Visser J, Voragen AGJ, eds.), Elsevier, Amsterdam, The Netherlands, 1996, pp. 723-730. 

Christensen TMIE, Nielsen JE, Kreiberg JD, Rasmussen P, Mikkelsen JD. Pectin methylesterase from orange fruits characterization and localization by in situ hybridization and immunohistochemistry. Planta 1998 206 493-503. 

Storey, R. and Treeby, M.T. 1994. The morphology of epicuticular wax and albedo cells of orange fruit in relation to albedo break-down. J. Horticult. Sci. 69, 329-338. 

Fig. 2.72. Examples of HPLC chromatograms (a) calibration standards (b) commercial orange fruit juice sample (c) hand-sqeezed grapefruit juice sample. Peaks (d) diosmin (h) hesperidin (n) naringin (IS) rhoifolin (internal standard). Reprinted with permission from. F. I. Kanaze et al. [190],...

In winter, the seed eapsules of this evergreen perennial split to reveal bright orange fruits. The eommon name refers only to the unpleasant smell of the leaves when erushed. 

Lo Piero AR, Puglisi I, Rapisarda P, Petrone G (2005) Anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage. J Agric Food Chem 53(23) 9083-9088... 

Cotroneo PS, Russo MP, Ciuni M, Recupero GR (2006) Quantitative real-time reverse transciiptase-PCR profiling of anthocyanin biosynthetic genes during orange fruit ripening. J Amer Soc Hort Sci 131(4) 537-543... 

Lang YQ, Yanagawa S, Sasanuma T, Sasakuma T (2004) Orange fruit color in Capsicum due to deletion of capsanthin-capsorubin synthesis gene. Breed Sci 54 33-39... 

Foods high in vitamin A yellow and dark green vegetables, yellow and orange fruits,... 

Citrus deliciosa Tenore C. nobilis Lour. Jiu Pi (Orange) (fruit skin) Vitamin A, and C, hesperidin, limonene, citral, methyl anthranilate.49 Stomachic, digestant, expectorant, antitussive, antiemetic. 

Poncirus trifoliata Rafin Gou Gi (Trifoliate orange) (fruit) Poncirin, limonin, imperatorin, bergapten, neohesperidin, citrifoliol, myrcene, camphene, gamma-terpinene.33 Treat gastric pain, constipation, and prolapse of the uterus or rectum. 

The major dietary sources of retinol are dairy products, eggs and liver, while important sources of /3-carotene are spinach and other dark-green leafy vegetables, deep orange fruits (apricots, cantaloupe) and vegetables (squash, carrots, sweet potatoes, pumpkin). The richest natural sources of vitamin A are fish liver oils, particularly halibut and shark. 

For coumarins in orange fruits (115), the HPLC used a Zorbax Rx C8 (250-mm X 4.6-mm ID, 5 fim) column maintained at 25°C, and analysis was performed by binary-gradient elution using 0.1% HOAc in acetonitrile (eluent A) and 0.1% HOAc in HzO (eluent B). In the author s lab, standard coumarins could be separated by isocratic elution on Zorbax Rx C8 column with acetonitrile-0.1% HOAc in water (35 65) at 1.0 ml/min, as presented in previous work (1). The eluate from the column was passed to a UV detector (UV 330 nm) and then into a fluorescence detector (excitation at 340 nm, emission at 425 nm). As for the specificity, some of the coumarins do not have native fluorescence. Nine coumarins are separated under UV 330 nm, and three coumarins could not be detected with fluorescence detection. Detailed conditions for coumarin analysis in foods and absorption spectra of coumarins obtained by online diode array detector with HPLC were presented by Lee and Widmer (1). Since coumarins exhibit strong absorption in the ultraviolet region, absorption at approximately 313 nm has been used to estimate the dilution of cold-pressed lemon oil with distilled oil (12). Analysis of umbelliferone (7-hydroxy-coumarin) and scopoletin (6-methoxy-7-hydroxycoumarin) in citrus fruits was performed using... 

Part of herb used The seeds, which are purple-brown to black with a characteristic white eye , contained within a red-orange fruit about the size of a hazelnut. Main actives Guarana has the highest known caffeine content of any herb at 3.6-5.8%. It also contains small amounts of theophylline and theobromine, the other stimulant purine alkaloids similar to caffeine. Besides these, guarana contains about 12% tannins and some saponins. 

Citrus fruits, especially certain of their component parts, constitute one of the richest sources of pectin. On a dry weight basis, as much as 30% of orange fruit albedo may be pectin (8). The rag, comprising the fruit core and segment membranes after juice extraction, is also a rich source. Since pectin is a cell wall component, it follows that comparatively little would be present in juice expressed from fruit. For example, concentrations ranging from 0.01 to 0.13% in orange juice have been reported (15). Much of this would be present as cell wall fragments and particulate material in juice pulp and cloud. 

Versteeg, C. Pectinesterases from the orange fruit - their... 

Orange fruit juice drink (0 % fat) 0.2 vom Bruck, Hammerschmidt, 1977... 

Iboga is frequently cultivated in villages of Gabon as a decorative shrub, producing a yellowish- or pinkish-white flower. This flower often grows from the same point with pairs of leaves and branches. The plant yields a small, oval, yellowish-orange fruit—about the size of an olive with an edible sweet pulp—which doesn t contain ibogaine but has sometimes been used as a medicine "for barrenness in women. ... 

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