Oral cells

Schwartz JL, Baker V, Larios E and Chung FL. 2005. Molecular and cellular effects of green tea on oral cells of smokers a pilot study. Mol Nutr Food Res 49(1)43—51. 

VII. EFFECTS OF PHENOLS AGAINST BACILLUS SUBTILIS (M45) (REC-ASSAY), HUMAN ORAL CELLS, AND HIV-INFECTED MT-4 CELLS... 

LED, lowest effective dose HID, highest ineffective dose in-vitro tests, ug/nil, in-vivo tests, mg/kg bw/day ip, intraperitoneal po, oral ° Cells pre-incubated for 24 h with 2 uM dexamethasone, an inducer of liver-specific cytochrome P450 isoforms results were negative without this pretreatment. 

MKC-1 [25] a bisindolylmaleimide compound, was identified as an oral cell cycle inhibitor that induces apoptosis in cancer cells by targeting tubulin and importin beta. It was evaluated in clinical trials for the potential treatment of breast cancer, non-smaU-cell lung cancer, leukemia, and ovarian cancer, but it was suspended after phase II studies. 

Fourth, on the antitumor activity) for the tumor cells, the hypophasic orange extract (PM3) and hypophasic apple extract (PM4) showed slightly their higher cytotoxicity against four the human tumor cells such as squamous cell carcinoma HSC-2, HSC-3, submandibular gland carcinoma HSG and human promyelocytic leukemic HL-60 cells when compared to their cytotoxic activity of three normal human oral cells such as gingival fibroblast HGF, pulp cell HPC and periodontal ligament fibroblast HPLF. 

For the initial studies of oral mucosa cells, normal cytological samples were harvested from laboratory volunteers, whereas clinical samples were collected at TMC, as described in Section 5.2.1.1, and immediately inserted into a vial filled with SurePath fixative. Data acquisition was carried out via the PapMap approach as discussed in Section 5.2.3.2 thus, for the first time, thousands of cellular spectra were available for statistical analysis. This large number of cells allowed several ancillary studies, for example, the immediate effects of smoking and over-the-counter medication on the spectra of oral cells, as well as the dependence of spectral characteristics on the site of cell collection. It was found that cells from the top of the tongue, the mouth floor, and the inside of the cheeks had slightly different spectral characteristics, which could be attributed to some different expression of proteins in these cells. Cells from the tongue, for example, had shghtly different... 

Norton, L. A., K. L. Andersen, D. Arenholt-Bindslev, L. Andersen, and B. Melsen. 1995. A methodical study of shape changes in human oral cells pertmbed by a simulated orthodontic strain in vitro. Arch Oral BioHO 863-72. 

Figure Bl.24.17. An example of scanning transmission ion microscopy (STIM) measurements of a human oral cancer cell. The different images indicate different windows in the energy of transmitted helium ions as indicated in the figure. White indicate areas of high counts. The teclmique offers a thickness scan through the sample, and, in this case, the cell walls of one specific cell can be seen in the areas dominated by thicker structures (data from C A Pineda, National Accelerator Centre, Fame, South Africa).

Liver and Gallbladder. High dosages of oral estrogens have been reported to increase the risk for jaundice, cholestatic hepatitis, gallstones, and hepatic vein blood clots. Estrogens promote the development of hepatic neoplasms associated with increased hepatic cell regenerative activity (186,187). 

A number of studies have shown that vitamins moderate the induction of chromosomal aberrations by radiation. Vitamins C and E given orally to mice either 2 h before, immediately after, or 2 h after 1 Gy (100 rad) of y-ray TBI significantly reduce the frequencies of micronuclei and chromosomal aberrations in BM cells. Vitamin E is the more effective (95). Administration of vitamins C and E within 5 min of irradiation is as effective as pretreatment. Protection by vitamin C has also been shown in humans. Whereas chronic treatment of rats using vitamin C (100 or 300 mg/(kg/d)) for six months prior to TBI protects against chromosomal aberrations, vitamin E is not radioprotective in this setting (96). 

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