Oral mucosal cell sheets

Oral mucosal cell sheets are also cfinically applied to the treatment of esophageal ulcerations using an endoscopic transplantation method (Kanai, Yamato, Ohki, Yamamoto, Okano, 2012 Ohki et al., 2006, 2012). Removal of large esophageal cancer tissue section by endoscopic submucosal dissection causes postoperative inflammation and stenosis from a lack of an epithelial layer. After the transplantation... 

A successful clinical study was conducted by using limbal-derived cell sheets for the treatment of patients suffering from unilateral total corneal stem-cell deficiencies [18], which resulted from alkali burns or Stevens-Johnson syndrome. In the case of bilateral total comeal stem-cell deficiencies, an autologous oral mucosal epithelial cell sheet was transplanted onto the ocular surface [19]. A clinical trial in France for the treatment of a bilateral limbal stem-cell deficiency had been conducted by using autologous oral mucosal epithelial cell sheets [20]. Through this clinical trial, the treatment was safe and effective in 25 cases with a 1-year follow-up, although two patients experienced serious adverse events, which were not related to the transplantation of the cell sheets. 

Esophagus Prevention of stenosis after endoscopic submucosal dissection of esophageal cancer Barrett s esophagus Oral mucosal epithelial cell sheet [22] Tokyo Women s Medical University (Japan) Karolinska Institute (Sweden) Started in April 2008 Started in April 2012... 

Ohki T, et al. Treatment of oesophageal ulcerations using endoscopic transplantation of tissue-engineered autologous oral mucosal epithehal cell sheets in a canine model. Gut 2006 55 1704-10. 

Burillon, C., Huot, L., Justin, V., Nataf, S., Chapuis, R, DecuUier, E., et al. (2012). Cultured autologous oral mucosal epithelial cell sheet (CAOMECS) transplantation for the treatment of comeal limbal epithelial stem cell deficiency. Investigative Ophthalmology and Visual Science, 53, 1325-1331. 

Murakami, D., Yamato, M., Nishida, K., Ohki, T., Takagi, R., Yang, J., et al. (2006a). The effect of micropores in the surface of temperature-responsive culture inserts on the fabrication of transplantable canine oral mucosal epithehal cell sheets. Biomateriais, 27, 5518-5523. 

Nishida, K., Yamato, M., Hayashida, Y, Watanabe, K., Yamamoto, K., Adachi, E., et al. (2004b). Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. New England Journal of Medicine, 351, 1187-1196. 

Watanabe, E., Yamato, M., Shiroyanagi, Y, Tanabe, K., Okano, T. (2011). Bladder augmentation using tissue-engineered autologous oral mucosal epithelial cell sheets grafted on demucosalized gastric flaps. Transplantation, 91, 700-706. 

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