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Of NADH

Step 2 The ketone carbonyl of the acetoacetyl group is reduced to an alcohol function This reduction requires NADPH as a coenzyme (NADPH is the phosphate ester of NADH and reacts similarly to it)... [Pg.1076]

Two or more linked enzyme reactions can lead to a change in the concentration of NADH or NADPH that is equivalent to the concentration of the original analyte. The reference glucose measurement using hexokinase [9001-51-8] and glucose-6-phosphate dehydrogenase [9001-40-5] is an example ... [Pg.38]

Cltra.te The citrate reaction is followed by monitoring the decrease in the concentration of NADH. Oxaloacetate instantiy decarboxylates to pymvate. [Pg.39]

The loss of NADH is followed for determination of the en2yme creatine kinase. [Pg.39]

Laetate The formation of NADH is followed by measuring the absorbance at 340 nm. [Pg.39]

Enzymes, measured in clinical laboratories, for which kits are available include y-glutamyl transferase (GGT), alanine transferase [9000-86-6] (ALT), aldolase, a-amylase [9000-90-2] aspartate aminotransferase [9000-97-9], creatine kinase and its isoenzymes, galactose-l-phosphate uridyl transferase, Hpase, malate dehydrogenase [9001 -64-3], 5 -nucleotidase, phosphohexose isomerase, and pymvate kinase [9001-59-6]. One example is the measurement of aspartate aminotransferase, where the reaction is followed by monitoring the loss of NADH ... [Pg.40]

Oxidation of P-nicotinamide adenine dinucleotide (NADH) to NAD+ has attracted much interest from the viewpoint of its role in biosensors reactions. It has been reported that several quinone derivatives and polymerized redox dyes, such as phenoxazine and phenothiazine derivatives, possess catalytic activities for the oxidation of NADH and have been used for dehydrogenase biosensors development [1, 2]. Flavins (contain in chemical structure isoalloxazine ring) are the prosthetic groups responsible for NAD+/NADH conversion in the active sites of some dehydrogenase enzymes. Upon the electropolymerization of flavin derivatives, the effective catalysts of NAD+/NADH regeneration, which mimic the NADH-dehydrogenase activity, would be synthesized [3]. [Pg.363]

Discuss the cycling of NADH and NAD in glycolysis and the related fermentation reactions. [Pg.637]

This is a good point to pause in our trip through the TCA cycle and see what has happened. A two-carbon acetyl group has been introduced as acetyl-CoA and linked to oxaloacetate, and two COg molecules have been liberated. The cycle has produced two molecules of NADH and one of GTP or ATP, and has left a molecule of succinate. [Pg.653]

NAD (P) " -dependent enzymes are stereospecific. Malate dehydrogenase, for example, transfers a hydride to die pro-/ position of NADH, whereas glyceraldehyde-3-phosphate dehydrogenase transfers a hydride to die pro-5 position of the nicotinamide. Alcohol dehydrogenase removes a hydride from the pro-i position of edianol and transfers it to die pro-i position of NADH. [Pg.656]

The half-reactions and reduction potentials in Table 21.1 can be used to analyze energy changes in redox reactions. The oxidation of NADH to NAD can be coupled with the reduction of a-ketoglutarate to isocitrate ... [Pg.678]

As we have seen, the metabolic energy from oxidation of food materials—sugars, fats, and amino acids—is funneled into formation of reduced coenzymes (NADH) and reduced flavoproteins ([FADHg]). The electron transport chain reoxidizes the coenzymes, and channels the free energy obtained from these reactions into the synthesis of ATP. This reoxidation process involves the removal of both protons and electrons from the coenzymes. Electrons move from NADH and [FADHg] to molecular oxygen, Og, which is the terminal acceptor of electrons in the chain. The reoxidation of NADH,... [Pg.679]

Although the precise mechanism of the NADH-UQ reductase is not known, the first step involves binding of NADH to the enzyme on the matrix side of the inner mitochondrial membrane, and transfer of electrons from NADH to tightly bound FMN ... [Pg.682]

FIGURE 21.33 The glycerophosphate shuttle (also known as the glycerol phosphate shuttle) couples the cytosolic oxidation of NADH with mitochondrial reduction of [FAD]. [Pg.703]

The second electron shuttle system, called the malate-aspartate shuttle, is shown in Figure 21.34. Oxaloacetate is reduced in the cytosol, acquiring the electrons of NADH (which is oxidized to NAD ). Malate is transported across the inner membrane, where it is reoxidized by malate dehydrogenase, converting NAD to NADH in the matrix. This mitochondrial NADH readily enters the electron transport chain. The oxaloacetate produced in this reaction cannot cross the inner membrane and must be transaminated to form aspartate, which can be transported across the membrane to the cytosolic side. Transamination in the cytosol recycles aspartate back to oxaloacetate. In contrast to the glycerol phosphate shuttle, the malate-aspartate cycle is reversible, and it operates as shown in Figure 21.34 only if the NADH/NAD ratio in the cytosol is higher than the ratio in the matrix. Because this shuttle produces NADH in the matrix, the full 2.5 ATPs per NADH are recovered. [Pg.704]

Note The.se P/O ratios of 2.5 and 1.5 for mitochondrial oxidation of NADH and [FADHg] are consen.sns values. Because diey may not reflect actual values and because these ratios may change depending on metabolic conditions, the.se estimates of ATP yield from glucose oxidation are approximate. [Pg.705]

Consider the oxidation of NADH by molecular oxygen as carried out via the electron transport pathway... [Pg.706]

NADPH can be produced in the pentose phosphate pathway as well as by malic enzyme (Figure 25.1). Reducing equivalents (electrons) derived from glycolysis in the form of NADH can be transformed into NADPH by the combined action of malate dehydrogenase and malic enzyme ... [Pg.805]

Synthesis and reactions of NADH and NADPH model compounds with interconversion of central and axial chirality 97YGK132. [Pg.238]

Dihydropyridines not only are intermediates for the synthesis of pyridines, but also are themselves an important class of N-heterocycles an example is the coenzyme NADH. Studies on the function of NADH led to increased interest in the synthesis of dihydropyridines as model compounds. Aryl-substituted dihy-dropyridines have been shown to be physiologically active as calcium antagonists. Some derivatives have found application in the therapy of high blood pressure and angina pectoris. For that reason the synthesis of 1,4-dihydropyridines has been the subject of intensive research and industrial use. The Hantzsch synthesis has thus become an important reaction. [Pg.153]

The first step in the biological degradation of lysine is reductive animation with a-ketoglutarate to give saccharopine. Nicotinamide adenine dinucleotide phosphate (NADPH), a relative of NADH, is the reducing agent. Show the mechanism. [Pg.1059]

Addition of the prc-R hydrogen of NADH takes place on the Re face of pyruvate. [Pg.1269]

During dtric add production there is massive generation of NADH but little demand for ATP. Thus the situation could quickly arise where there is no further ADP available for oxidative phosphorylation within the cells. This means that the electron transport chain cannot operate and no further oxidation of NADH can occur. [Pg.130]


See other pages where Of NADH is mentioned: [Pg.646]    [Pg.539]    [Pg.349]    [Pg.551]    [Pg.572]    [Pg.590]    [Pg.609]    [Pg.631]    [Pg.641]    [Pg.651]    [Pg.656]    [Pg.667]    [Pg.673]    [Pg.699]    [Pg.702]    [Pg.704]    [Pg.706]    [Pg.825]    [Pg.305]    [Pg.724]    [Pg.1074]    [Pg.56]    [Pg.121]    [Pg.125]    [Pg.130]    [Pg.130]   
See also in sourсe #XX -- [ Pg.368 ]

See also in sourсe #XX -- [ Pg.368 ]




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5-Deazaflavin nonenzymatic oxidation of NADH

Absorption Spectrum of NADH

Acid catalysed decomposition of NADH

Decay of NADH

Electrocatalytic oxidation of NADH

Glyceraldehyde 3-phosphate dehydrogenase formation of NADH

Mediators for Electrochemical Oxidation of NADH

NADH

Oxidation of NADH

Regeneration of NADH

Regeneration of the cofactor NADH

The configuration of NADH and NADPH

The oxidation of NADH

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