Decay of NADH

CrCAHERTZ FREQUENCY-DOMAIN FLUOROMETRY Advanced Topic  

What range of frequencies can be expected with a pulsed laser light source and an MCP PMT detector For Lor tzian-sh ed pulses, the harmonic content decreases to one-half of the low-frequency intensity at a frequent 0)2 = 2 In 2/Ar, where Ar is the pulse width.For 5-ps Hilses. the harmonics extend to 280 GHz, hi er than tte upper frequency limit of any available detector. Hence, for the foreseeable ftiture, the measurements will be limited by the detector. 

FigiNV S.39. External cron oonrrtadoo of an MCP PMT. From Ref. lOi. R xinied widi peRnladoo fron tfie aeiicMi Institute of Pl sics, Cof ghtO l9S6. 

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Figure 20.12 (A) Fluorescence spectra of NADH in the presence of different concentrations of gold nanospheres (OD=1.0). Inset the fluorescence decay of NADH at 460 nm in the presence of different concentrations of gold nanospheres ( exci = 335 nm) (B) Absorption spectra of NADH (O.OSmM) in the presence of different concentrations of gold nanospheres (OD=1.0) Inset absorption spectra of NADH after subtraction of absorptions from gold nanospheres (C) Fluorescence spectra of NADH in the presence of different concentrations of gold nanorods (OD=1.0). X,exci = 325 nm Inset the fluorescence decay of NADH at 460 nm in the presence of different concentrations of gold nanorods ( exci = 335 nm) (D)Absorption spectra of NADH (O.OSmM) in the presence of different concentrations of gold nanorods (OD=1.0). Cited from reference 52.

Hgure 5.43. Freqiiency lonuin uSeiisity decay of NADH measuied op to 2 GHz Rom Ref. 3. 

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