Octanesulfonate sodium

Substrates (p-octopamine, dopamine, or 5-hydroxytryptamine) were separated from their N-acetylated products on an Ultrasphere I.P. C18 column (4.6 mm x 250 mm, 5 pm). The mobile phase was comprised of 75 mAf monobasic sodium phosphate, 1 pAf EDTA, 0.35 mAf 1-octanesulfonate (sodium salt), 11% methanol, and 4% acetonitrile. Coulometric detection was used. Detection of p-octopamine and its N-acetylated product was achieved at a potential of +0.75 V, while dopamine, 5-hydroxytryptamine and their products were detected at +0.50 V. 

EINECS 226-195-4 NSC 2738 1-Octanesulfonic acid, sodium salt Sodium 1-octanesulfonate Sodium ootane-1-sulphonate monohydrate Sodium octanesulfonate Sodium octylsulfonate. 

Sodium 1-octanesulfonate [5324-84-5] M 216.2. Recrystd from absolute EtOH. 

Favaro and Fiorani [34] used an electrode, prepared by doping conductive C cement with 5% cobalt phthalocyanine, in LC systems to detect the pharmaceutical thiols, captopril, thiopronine, and penicillamine. FIA determinations were performed with pH 2 phosphate buffer as the carrier stream (1 mL/min), an injection volume of 20 pL, and an applied potential of 0.6 V versus Ag/AgCl (stainless steel counter electrode). Calibration curves were developed for 5-100 pM of each analyte, and the dynamic linear range was up to approximately 20 pM. The detection limits were 76, 73, and 88 nM for captopril, thiopronine, and penicillamine, respectively. LC determinations were performed using a 5-pm Bio-Sil C18 HL 90-5S column (15 cm x 4.6 mm i.d.) with 1 mM sodium 1-octanesulfonate in 0.01 M phosphate buffer/acetonitrile as the mobile phase (1 mL/min) and gradient elution from 9 1 (held for 5 min) to 7 3 (held for 10 min) in 5 min. The working electrode was maintained at 0.6 V versus Ag/AgCl, and the injection volume was 20 pL. For thiopronine, penicillamine, and captopril, the retention times were 3.1, 5.0, and 11.3 min, and the detection limits were 0.71, 1.0, and 2.5 pM, respectively. 

Figure 4.12 Effect of counter-ions and copper on the retention of amino acids. Column, octadecyl-bonded silica gel, 25 cm x 4.6 mm i.d. eluent, 0.01 M sodium acetate buffer (pH 5.6) containing 1.2 mM sodium octanesulfonate (Oc) andj or 0.1 mM copper acetate (Cu) flow rate, 1ml min-1 detection, UV 220 nm. Compounds Glu, glutamic acid, Asp, aspartic acid.

More recently [635], a unique extraction step in supplemented foods, by using hot water and a precipitation solution, following by HPLC-ELD/UV analysis has been performed for the simultaneous determination of pyridoxine, thiamine, riboflavin, niacin, pantothenic acid, folic acid, cyanoco-balamin, and ascorbic acid. The mobile phase consisting of phosphate buffer and methanol has been modified in order to perform ion-liquid chromatography by adding l-octanesulfonic acid sodium salt. Furthermore, triethylamine has been also added to improve peak symmetry. 

Measurement of Dorzolaniide in Plasma IV Beckman RP-8 (250 X 4.6 mm, 5pm) Solvent A Acetonitrile Solvent B Sodium salt of octanesulfonic acid in 0.085% phosphoric acid in water 25% solvent A 75% solvent B 252 nm 13. 

Increased retention of the analytes can also be achieved by addition of various ion-pair reagents in the mobile phase Tetrabutylammonium cations have typically been used as counter ions, at around pH 6.5, to increase retention and improve the selectivity in the analysis of monobasic penicillins (105, 123). Alkyl-sulfonic acids have been also used to improve the separation of -lactams bearing an amine function in their side chain or having a neutral side chain. Heptanesul-fonic acid (80, 103), decanesulfonic acid (87, 93, 106), dodecanesulfonic acid (77, 107-110), or mixtures of octanesulfonic and dodecanesulfonic acids (73, 75, 78, 79) constitute the principal alkylsulfonic acids used in -lactam analysis. In some applications, heptafluorobutyric acid (74, 76) or sodium thiosulfate (90, 112, 115, 116, 121, 122) has also been used as an ion-pairing reagent. 

Figure 26-9 Principle of ion-pair chromatography. The surfactant sodium octanesulfonate added to the mobile phase binds to the nonpolar stationary phase. Negative sulfonate groups protruding from the stationary phase then act as ion-exchange sites for analyte cations such as protonated organic bases, BH+.

Hernandez-Jover et al. (72) derived an improved analytical method from the HPLC procedure setup they developed in 1995 (73) for the determination of BAs in fish. The method consists of the extensive extraction with HC104, ion-pair (with sodium octanesulfonate) RP-HPLC separation, postcolumn derivatization with PA/ME, and spectrofluorimetric detection. Determination limits were up to 1.5 mg/kg. In particular, His, Tyr, Phe, Ser (serotonine), Cre (creatinine), Try, Oct (octopamine), Dop (dopamine), Cad, Put, Agm (agmatine), Spm, and Spd were studied in pork and beef meat, fresh, cooked, or ripened. Tyramine, His, Put, Cad, and Try levels were... 

Fig. 3. Trace determination of some inorganic and organic anions in pure water, after an electrophoretic enrichment at 5 kV for 45 s with an addition of 75 p,M octanesulfonate to the sample [42]. The electrolyte 10 mM sodium chromate and 0.5 mM OFM-BT (a surfactant used as the EOF modifier), adjusted to pH 8 with sulfuric acid 15 kV 60 cmX75 fjim I.D. capillary, distance to detector, 52 cm UV photometric detection at 254 nm. Anions (concentrations in mg/1) 1, chloride (3.5) 2, sulfate (4.8) 3, nitrate (6.2) 4, oxalate (5) 5, fluoride (1.9) 6, formate (5) 7, phosphate (3.2) 8, acetate (5) 9, propionate (5).

Fig. 6.22. Electrochromatographic separation of Gly-Tyr (1), Val-Tyr-Val (2), methionine enkephalin (3), and leucine enkephalin (4) on monolithic methacrylate capillary column with a pore size of 492 nm. (Reprinted with permission from [55]. Copyright 1999 Wiley-VCH). Conditions Mobile phase 10% of aqueous 10 mmol/L sodium 1-octanesulfonate and 90% of a 2 8 mixture of 5 mmol/L phosphate buffer pH=7.0 and acetonitrile. UV detection at 215 nm. Total sample concentration 1 mg/mL.

L-Dopa was separated on a Cosmosil 5 Qg column (4.6 mm X 250 mm). The mobile phase contained 90 mM sodium acetate, 35 mAf citric acid, 130 fiM disodium EDTA, and 230 fiM sodium n-octanesulfonate in 10.5%... 

The reactants and products were separated on an MOS Hypersil column (4.6 millimeters x 200 mm, 5 /urn). The mobile phase was composed of a 90 10 mixture of solvent A, consisting of 0.1 M sodium acetate, 0.02 M citric acid, 0.93 mM sodium octanesulfonate, and 0.12 mM disodium EDTA (pH 4.6), and solvent B, methanol UV detection was used, with the optimal wavelength being 258 nm for the adenoxyl derivatives and 279 nm for adrenaline and noradrenaline. Quantitation was normally based on the S-adenosyl-L-homocysteine formed. 

Kochhar et al. (1989) characterized an assay for glutamate decarboxylase activity. Glutamate and 4-aminobutyrate were separated on a Nucle-osil Q column. The mobile phase was 13 mAf trifluoroacetate and 1 mAf 1-octanesulfonate. Detection was by postcolumn derivatization with o-phthaldialdehyde reagent (1 mL/min) mixed with the column eluate (also 1 mL/min). The Teflon reaction coil (3 m x 0.3 mm) was kept at room temperature. The o-phthaldialdehyde reagent was prepared by dissolving 800 mg of o-phthaldialdehyde in 20 mL of ethanol plus 2.5 mL of 2-mercaptoethanol and mixing with 980 mL of 0.4 Af sodium borate (pH 9.7) and 3 mL Brij 35. The fluorometer was set to give excitation at 350 nm and emission was measured at 450 nm. 

Following derivatization with o-phthalaldehyde, TV1 -acetylspermidine, and putrescine are separated on a Beckman Ultrasphere ion-pair column (4.6 mm x 250 mm, 5 /urn). Solvent A was composed of 0.1 M sodium acetate and 10 mM octanesulfonic acid adjusted to pH 4.5 with acetic acid. Solvent B contained 0.2 M sodium acetate (pH 4.5)-acetonitrile (10 3, v/v) with 10 mM octanesulfonic acid. A linear gradient from 35% A and 65% B to 100% B was achieved in 10 minutes, followed by continued flow of solvent B for 15 minutes. The flow rate was 1 mL/min. Postminutes, column derivatization of polyamines with o-phthalaldehyde was accomplished with a pump. Fluorescence detection was used, with excitation and emission wavelengths of 340 and 455 nm. 

Fig. 2 CCC separation of water-soluble vitamins by cross-axis CPC. Experimental conditions sample, riboflavin sodium phosphate (2.5 mg)-I-cyanocobalamin (2.5 mg)-i-pyridoxine hydrochloride (2.5 mg)-i-thiamine nitrate (2.5 mg) solvent system, 1-butanol and aqueous 0.15 M monobasic potassium phosphate containing 1.5% of 1-octanesulfonic acid sodium salt mobile phase, lower phase flow rate, 0.2 mL/min. For other experimental conditions, see Fig. 1 caption. SF = solvent front.

Counter ion C5, sodium 1-pentanesulfonate C6, sodium 1-hexanesulfonate C7, sodium 1-heptanesulfonate C8, sodium 1-octanesulfonate CIO, sodium decanesulfonate C12, sodium dodecyl sulfonate The mobile phase (a) C5-C8, 10 mM solution (pH 4.0) acetonitrile (67 33). (b) CIO, CI2, 10 mM solution (pH... 

Fig. 5-30. Separation of epinephrine, ephedrine, and opium alkaloids. - Separator column IonPac NS1 (10 pm) eluent 0.005 mol/L sodium octanesulfonate + 0.05 mol/L KH2P04 (pH 4.0) / acetonitrile (89 11 v/v) flow rate 1 mL/min detection UV (220 nm) injection volume 50 pL solute concentrations 10 mg/L epinephrine, 10 mg/L morphine sulfate, 20 mg/L ephedrine hydrochloride, and 20 mg/L codeine phosphate.

The various arylalkylamines and alkaloids can be separated with ion-pair chromatography by coating the stationary phase with sodium octanesulfonate. The effect of type and concentration of the ion-pair reagent on the retention of these compounds has been described in Section 5.3.1 at the diphenhydramine example. Fig. 5-30 reveals that under similar chromatographic conditions further arylalkylamines such as epinephrine and ephedrine can be separated in the presence of opium alkaloids such as morphine and codeine (3-monomethylester of morphine). 

Fig. 8.54. Analysis of sodium perborate by ion-exclusion chromatography. — Separator column IonPac ICE-AS1 eluent 0.0011 mol/L octanesulfonic acid flow rate 1 mL/min detection suppressed...

Sodium 1-octanesulfonate H2O 75524-54-57 M 216.2. RecrystaUise it from absolute EtOH. [Beilstein 4 IV 58.]... 

Sample preparation Condition a 1 mL 100 mg Sep-Pak Vac trifunctional C18 SPE cartridge with 1 mL MeOH and 1 mL buffer. 0.5 mL Plasma -I- 0.5 mL buffer, vortex, add to SPE cartridge, wash with 0.5 mL buffer, elute with 300 p-L MeOH 5 mM sodium octanesulfonate 20 80 adjusted to pH 8.50 with 4 M NaOH, inject a 130 pL aliquot of the eluate. (Procedure was automated (ASPEC system). Buffer was 5 mM sodium octanesulfonate adjusted to pH 2.85 with concentrated orthophosphoric acid.)... 

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