Nylon grid

SAN these blends are miscible. Commercial grade PVC, PMMA, and SAN were used as-received (Table 11.9). Polymer blends were made by using an internal mixer. Very thin samples (100 to 200 nm thick) were cut at room temperature using an ultramicrotome. The samples were mounted on copper or nylon grids and coated on one side with 5 to 20 nm of carbon to reduce charge and shrinkage during irradiation in the electron microscope. 

Filter debris on a nylon grid funnel (mesh size 1 mm). 

Brain slices were transferred and anchored to a recording chamber with a nylon grid. A peristaltic pump continually perfused the slices with ACSF (bubbled with carbogen) at a rate of 1-2 mL/min. The recording temperature was controlled at either 26 or 35 °C with an inline heating system. Slices were visualized with an upright microscope equipped with DIG optics and were displayed on a monitor using a CCD camera. 

The positive plates are siatered silver on a silver grid and the negative plates are fabricated from a mixture of cadmium oxide powder, silver powder, and a binder pressed onto a silver grid. The main separator is four or five layers of cellophane with one or two layers of woven nylon on the positive plate. The electrolyte is aqeous KOH, 50 wt %. In the aerospace appHcations, the plastic cases were encapsulated in epoxy resins. Most usehil cell sizes have ranged from 3 to 15 A-h, but small (0.1 A-h) and large (300 A-h) sizes have been evaluated. Energy densities of sealed batteries are 26-31 W-h/kg. 

A variety of formats for protein arrays are possible. For example, a set of antibodies can be gridded on a filter or slide and used to detect protein expression levels (Pandey and Mann, 2000). Another type of array consists of proteins from an organism arrayed directly on to a glass slide, nylon filter or in microtiter wells (MacBeath and Schreiber, 2000). This format could be used to map protein-protein interactions or to associate a catalytic function with a protein. 

Samples for infra-red absorption measurement were introduced between two rubbed nylon coated calcium fluoride substrates spaced 10 pm apart. To insure proper parallel alignment, samples were cooled at 0.02° C/minute from the isotropic to the Smectic C phase. The alignment was then checked using polarizing microscopy. Polarized IR spectra (32 scans per spectrum) were obtained using an FTIR spectrometer (IFS-66 Bruker, Pillerica, MA) equipped with a wire grid polarizer at a resolution of 2 cm"1. 

Geotextiles are available as mats, textiles, webs, nets, grids, and sheets. When retention of the contained material is desired synthetic polymers such as polypropylenes, polyesters, nylons, PEs, and PVCs are used because they resist rapid degradation. When only shortterm retention is needed natural materials such as cotton are used. Geotextiles are not always made from fibers, but include film materials such as PE and polypropylene sheets used to retain moisture but retard weed growth in gardens. 

A fully automated system uses either cDNA fragments of known genes or libraries of oligonucleotides, which are then spotted in duplicate onto hybridization filters (nylon membranes or PVDF) with a grid for guidance. When nylon membranes are used, these filters in microarrays can be used several times. There are two possible ways of applying the DNA to the filter (Cahill, 2001) ... 

Entrapment in polyacrylamide gel over a platinum grid matrix Glutaraldehyde-mediated reaction with a nylon-polyethyleneimine copolymer Reaction with macroporous aminated silo chrome coated with an acrolein-vinylpyridine copolymer Adsorption onto tannin coupled to amino hex y 1-cellulo se Entrapment in cellulose fibres Carbodi-imide-mediated reaction with chitosan... 

For blind nuclear injections, oocytes are placed into a petri dish containing a 1.0-mm nylon mesh (Small Parts Inc., Miami Lakes, FL, cat. Q-CMN-1000). The mesh can be attached to the bottom of the dish with a few drops of chloroform. The grid wells of the mesh are similar in dimension to the diameter of the oocyte and serve to hold the oocytes in place. The petri dish is filled with MBSH media and the oocytes are oriented under a stereomicroscope with a pair of blunt forceps in the grid wells, with the pigmented animal hemisphere at the top. The injection needle is inserted at the center of the pigmented hemisphere and the needle tip is driven into the oocyte a depth of approximately one-fifth the diameter of the oocyte. The success rate of blind injections depends greatly on practice, but eventually a nuclear hit-rate of 70-90% is obtainable. The best way to practice nuclear injections is to inject oocytes with a 20 mg/ml solution of blue dextram and determine if the blue color is associated with the nuclear or cytoplasmic fractions after oocyte dissections. It should also be noted that RNA can be injected directly into isolated nuclei devoid of cytoplasm (Terns and Dahiberg, 1994). 

PTA is known to react with monomer epoxy resins which extract the stain [73], precluding its use prior to epoxy embedding. Therefore, PTA stained material is usually either embedded in glycol methacrylate or polyester resins, or sections are post-stained in cured epoxies. PTA penetration is slow and about 100 pm penetration into a block of material can be expected. Sections on grids are immersed into the solution or placed on a droplet. Pretreatment in absolute ethanol increases exposure to the stain. Martinez-Salazar and Cannon [136] reported a new method of PTA staining of nylon 6 and nylon 6,6 using 2% PTA and 2% benzyl alcohol. Thin films on a specimen grid were floated on a drop of the mixed solution for 10 min and then washed in water several times. 

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