Primer design, automated

The widespread use of PCR amplification in microarray manufacture places much importance on the availability of informatics tools for automated primer design. A primer is a synthetic oligonucleotide that hybridizes to a complementary nucleic acid template, and expedites (primes) enzymatic synthesis by providing a starting point for enzyme. PCR primers bind to proximal and distal sites on cDNA templates, allowing target sequences... 

NMR spectrum of the product exhibited a perfect glycosylation on the designated polymer primer 3. This result suggests that the present method to use novel type of glycosyltransferase microarray chip might materialize automated microsynthesizer for glycoconjugates. 

RAPD is an easy and quick assay that requires low quantities of DNA (5-50 ng per reaction). The design of primers does not require sequence data since the primers sites are randomly distributed throughout the genome. Each reaction generates multiple bands that are often sufficient to reveal changes. Reading of RAPD patterns is amenable to automation. 

---