Duplex formation

Streptavidin-single-stranded DNA covalent conjugates were described as the building blocks for assembling nanostructured scaffolds [31], The amount and type of biotinylated ligands were used to modulate the affinity of duplex formation between solid-phase-bound nucleic acid templates and DNA-streptavidin conjugates. This system has been proposed for the design of fine-tuned sequence detection systems. 

Very recently the trans- [Pt(NH3)2Cl2] isomer has been shown to promote the formation of some highly unusual multiple strand nucleic acid structures (28). A parallel-stranded DNA duplex, psPtDNA, has been prepared which incorporates a frarcs-Pt(NH3)2+ crosslink. The pla-tinated strands were synthesized through the series of reactions illustrated in Fig. 3. The initial mono-functional G N7 adduct is formed at pH 3.5 by addition of rans-[Pt(NH3)2(H20)Cl]+ to the pyrimidine-rich sequence 5 -d(T4CT4G). After the pH is increased, normal anti-parallel duplex formation occurs upon addition of the complementary purine-rich strand, 5 -d(A4GA4G). This intermediate anti-parallel stranded... 

Queuing by the post office Building of an ant hill Numbering of book pages DNA duplex formation Oligomeric assembly of hemoglobin... 

One of the most intriguing biological supramolecular systems that should be examined by the enthalpy-entropy compensation theory is the duplex formation and intercalation of DNA and RNA. 

In the thermodynamic study of duplex formation, a variety of complementary pairs of relatively simple, well-defined oligonucleotides are employed, " while the intercalation thermodynamics was examined with more complex or natural DNA duplexes. " Typical intercalating agents examined are acridine orange, acriflabine, actinomycin, daunomycin, ethidium bro-... 

Pulse measurements. A self-complementary RNA with 12 base pairs was spin labelled at uridine near the middle of the strand.83 Three-pulse PELDOR gave an interspin distance of 35 2 A, which is in good agreement with molecular modelling. For a shorter self-complementary RNA, dipolar modulation was not observed, which was attributed to aggregation or weak duplex formation. 

DNA Renaturation Involves Duplex Formation from Single Strands Chromosome Structure... 

The duplex formation in reaction (16.24) involves two molecules and is described as a bimolecular process, or one that occurs with a molecularity of two. The formation of the hairpin structure in reaction (16.25), however, involves only a single molecule and the reaction is said to be unimolecular and has a molecularity of one. 

The guanine redox activity of the target DNA was used to detect the duplex formation by carrying out a square-wave voltammetric measurement (SQW). The results of the parameters optimisation for the probe concentration (40 pg/ml), immobilisation time (5 min by applying a positive potential of +0.5V vs. Ag/AgCl pseudo reference electrode) and hybridisation time (10 min without any potential) were very similar to those obtained in previous work [3]. 

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