Nucleic Acids Amplification

Recent advances in nucleic acid amplification and detection... 

Klevits, T., et al. (1991). NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection. J. Virol. Methods 35,273-286. 

Vora G.J, Meadors C.E., Stenger D.A., Andreadis J.D., Nucleic acid amplification strategies for DNA microarray-based pathogen detection, Appl. Environ. Microbiol. 2004 70 3047-3054. 

Alternative methods of diagnosis include enzyme immunoassay, DNA probes, and nucleic acid amplification techniques. 

Tests that allow rapid identification of chlamydial antigens in genital secretions are the direct fluorescent antibody test, the enzyme immunoassay (requires just 30 minutes for results), the DNA hybridization probe and nucleic acid amplification tests. 

Antibody capture of viruses can be used as a preparatory step in nucleic acid amplification techniques. Immunocapture of virus particles can be used to streamline and/or optimize the concentration, purification and specificity requirements of polymerase chain reaction assays. 

Nucleic acid amplification testing nucleic acid testing Nucleotide-binding domain... 

Nucleic acid probe methods which rely on the specificity of nucleic acid hybridization reaction, combined with the powerful methods of nucleic acid amplification, are expected to spawn many new diagnostic tests which may be useful in the detection and treatment of infectious diseases, cancer, and inherited disorders. The impact of development and applications of diagnostic tests based on nucleic acid probes and on Mab technology wiU be felt increasingly as deeper understanding of diseases at a molecular level is gained. 

Battle TJ, Golden MR. Evaluation of laboratory testing methods for Chlamydia trachomatis infection in the era of nucleic acid amplification. J Clin Microbiol 2001 39 2924-2927. 

All of these tasks can now be addressed with a family of novel tests collectively named product-enhanced reverse transcriptase (PERT) assays. PERT assays combine the broad detection range of RT tests with the high sensitivity of nucleic acid amplification procedures. PERT assays are based on the selective enhancement, by polymerase chain reaction (PCR) or one of the various... 

Wilding P. Nucleic Acid Amplification in Microchips, In Cheng JKL, ed. Biochip technology. Philadelphia Harwood Academic Publishers, 2001 173-84. 

Yuen PK, Kricka L, Fortina P, Panaro NJ, Sakazume T, Wilding P Microchip module for blood sample preparation and nucleic acid amplification reactions. Genome Res 2001 11 405-12. 

Polymerases catalyze the synthesis of complementary nucleic acid polymers using a parent strand as a template. In vitro, these enzymes can extend an oligonucleotide primer that is annealed to a template strand. Extension requires that the 3 OH of the extending end is free, and that nucleotide triphosphates (NTPs) are present. Extension stops if you rrm out of template or NTPs, or if no 3 OH groups are. available at the extending end. Thermostable polymerases, such as Thermus aquaticus (Taq) DNA polymerase, are essential reagents for the automation of many nucleic acid amplification procedures. 

Nucleic acid-based testing for infectious diseases, as for other clinical conditions, usually involves three major steps specimen processing, nucleic acid amplification, and... 

Baker CA, Cartwright CP, Williams DN> Nelson SM, Peterson PK. Early detection of central nervous system tuberculosis with the gen-probe nucleic acid amplification assay utility in an inner city hospital. Clin Infect Dis 2002 35 339-42. 

Centers for Disease Control and Prevention. Nucleic acid amplification tests for tuberculosis. MMWR 1996 45 950-2. 

Saldanha J, Lelie N, Heath A. Establishment of the first international standard for nucleic acid amplification technology (NAT) assays for HCV RNA. WHO Collaborative Study Group. Vox Sang 1999 76 149-58. 

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