Neurospora crassa purified preparations

Tryptophan synthetase has also been purified from several other sources, and Neurospora crassa appears to be a particularly convenient source for the large scale preparation of the enzyme [67]. The enzyme from the latter source has been used for detailed stereochemical studies particularly with respect to the events which occur at... 

Roseman et al. (1958) purified the enzyme from microbial and mammalian extracts and demonstrated that fructose-6-phosphate, and not glucose-6-phosphate, was the required substrate. Ghosh et al. (1969) purified the enzyme from Neurospora crassa, E. coli, and rat liver until it was free from phosphoglucose isomerase and glutaminase. Using this more purified enzyme preparation, fructose-6-phosphate was shown to be the required substrate, and a 1 1 stoichiometry was demonstrated for glutamine and fructose-6-phosphate. 

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