Nematodes identification

Table 2.1. Filarial nematodes infected with intracellular bacteria. The method of detection is shown. Neither electron microscopy nor the immuno-histochemical staining techniques used are to be regarded as Wolbachia specific (see note). Positive identification of intracellular bacteria as Wolbachia is shown only where PCR amplified products of rRNA or ftsZ genes have been sequenced. ...

Identification of Parasitic Nematodes and Study of Genetic Variability Using PCR Approaches... 

The accurate analysis of genetic variation in nematodes has major implications for parasite identification and for investigating population genetic structures (Lejambre, 1993 Grant, 1994). Conventional DNA methods have been valuable and their application has provided a wealth of molecular data (reviewed by McManus and Bowles, 1996), but little attention has been paid to their capacity to resolve sequence variation (reviewed by Gasser, 1997). For instance, PCR-RFLP analysis using (multiple) restriction endonucleases is useful, but sequence variation may go undetected as... 

Gasser, R.B. and Monti, J.R. (1997) Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA. Molecular and Cellular Probes 11,201-209. 

Grenier, E., Bonifassi, E., Abad, P. and Laumond, C. (1996) Use of species-specific satellite DNAs as diagnostic probes in the identification of Steinernematidae and Heterorhabditidae entomopathogenic nematodes. Parasitology 113, 483-489. 

Humbert, J.F. and Cabaret, J. (1995) Use of random amplified polymorphic DNA for identification of ruminant trichostrongylid nematodes. Parasitology Research 81, 1-5. 

Cookson, E., Blaxter, M. and Selkirk, M. (1992) Identification of the major soluble cuticular glycoprotein of lymphatic filarial nematode parasites (gp29) as a secretory homolog of glutathione-peroxidase. Proceedings of the National Academy of Sciences USA 89, 5837-5841. 

Selkirk, M.E., Nielsen, L., Kelly, C., Partono, F., Sayers, G. and Maizels, R.M. (1989) Identification, synthesis and immunogenicity of cuticular collagens from the filarial nematodes Brugia malayi and Brugia pahangi. Mokcular and Biochemical Parasitology 32, 229—246. 

Klingbeil, M.M., Walker, D.J., Arnette, R., Sidawy, E., Hayton, K, Komuniecki, P.R. and Komuniecki, R. (1996) Identification of a novel dihydrolipoyl dehydrogenase-binding protein in the pyruvate dehydrogenase complex of the anaerobic parasitic nematode, Ascaris suum. Journal of Biological Chemistry 271, 5451-5457. 

The Japanese people live in a delicate micr0eCOsystem that can be easily polluted by industrial chemical accidents and the use of persistent agricultural chemicals. An intensive scientific effort has led to the isolation and identification of biodegradable natural products for potential use in agriculture. These include microbial metabolites that have activity against plants, microorganisms, nematodes, and insects. 

Nomura H, Athauda SB, Wada H, Maruyama Y, Takahashi K, Inoue H (2006) Identification and reverse genetic analysis of mitochondrial processing peptidase and the core protein of the cytochrome bcl complex of Caenorhabditis elegans, a model parasitic nematode. J Biochem (Tokyo) 139 967-979... 

Gasser, R.B. (2001) Identification of parasitic nematodes and study of genetic variability using PCR approaches. In Kennedy, M. and Harnett, W. (eds) Parasitic Nematodes Molecular Biology, Biochemistry and Immunology. CAB International, Wallingford, UK, pp. 53-82. 

Britton, C., Redmond, D.L., Knox, D.P., McKerrow, J.H. and Barry, J.D. (1999) Identification of promoter elements of parasite nematode genes in transgenic Caenorhabditis elegans. Molecular and Biochemical Parasitology 103, 1 71-1 81. 

Van Rossum, A.J., Brophy, P.M., Tait, A., Barrett, J. and Jefferies, J.R. (2001) Proteomic identification of glutathione S-transferases from the model nematode Caenorhabditis elegans. Proteomics 1, 1463-1468. 

Pyrowolakis, A., Westphal, A., Sikora, R.A,. Becker, J.O. Identification of root-knot nematode suppressive soils. Appl Soil Ecol 2002 19 51-56. 

Lehtonen, M., Reisner, K., Auriola, S., Wong, G., and Callaway, J. (2008). Mass-spectrometric identification of anandamide and 2-arachidonoylglycerol in nematodes. Chem. Biodwers. 5, 2431. 

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