Neisseria meningitidis capsular polysaccharide

Combination of pyrolysis and m.s. has been found to enable rapid analysis of sialic acids and other carbohydrates (for ng quantities of oligosaccharides and polysaccharides). Thus, the presence of (2— 8) and (2—>9) linkages in Neisseria meningitidis capsular polysaccharides has been confirmed.154... 

J. Haverkamp, H.L.C. Meuzelaar, E.C. Beuvery, P.M. Boonekamp and R.H. Tiesjema, Characterization of Neisseria meningitidis capsular polysaccharides containing sialic acid by... 

Neisseria meningitidis Types A and Ct, Cultures of N. meningitidis of serotypes A and C 1 Precipitation with hexadecyl-trimethyammonium bromide 2 Solubilization and purification 3 Blending 4 Freeze-drying Estimation of capsular polysaccharide content ... 

The first report of the occurrence of KDO in a structure other than LPS was that of Taylor47 on the capsular polysaccharide (K-antigen) of a clinical isolate, Escherichia coli LP1092. Almost simultaneously, Bhattachaijee and coworkers83 described an exopolysaccharide from Neisseria meningitidis serogroup 29e. This material contains KDO... 

Epimerization at C-2 of UDP-GlcNAc, leading to the UDP derivative of 2-acetamido-2-deoxy-D-mannose, was also observed115 118 the monosaccharide was identified as a constituent of several capsular polysaccharides from Streptococcus pneumonia119-122 and Neisseria meningitidis group A (Ref. 123), the teichuronic acid of Bacillus cereus,124 and the O-specific... 

The monomeric mechanism of chain assembly is characteristic for homopolysaccharides, which constitute the most difficult case for biosynthetic studies, as accumulation of intermediates could not be induced by removal of one of the glycosyl donors required for chain elongation. Among these polymers, the most extensive information has been obtained for polymers of JV-acetylneuraminic acid, namely, the capsular polysaccharides (36 and 37) of E. coli K1 (Ref. 354) and Neisseria meningitidis type c (Ref. 355), respectively. CMP-NeuAc serves as the glycosyl donor in the formation... 

Not all capsular polysaccharides of uropathogenic and invasive coli bacteria contain KDO. Some of the non-KDO capsular polysaccharides are shown in Figure 12 (11-16). These are the Kl, K2, K5 and K51 polysaccharides. The Kl polysaccharide is a poly-a-2.8-N-acetyl-neuraminic acid and has been known as colominic acid since many years (11). Its structure which is identical with that of the capsular antigen of Neisseria meningitidis B has first been reported by McGuire and Binkley... 

In both S. pneumoniae and N. meningitidis, the thickness of the capsule has been shown to vary at different points in infection. In Neisseria meninigitidis decreased capsule production enhances tissue invasion, while increased capsule production is essential for survival in systemic infections [349]. Likewise, studies in pneumococci have suggested that the capsule prevents bacterial adhesion to epithelial cells, as well as to endothelial cells [350,351,352]. Bacteria producing less capsular polysaccharide more efficiently colonize mucosal surfaces, while those producing more capsule are more virulent in systemic infections [350,353]. 

Meningococcal vaccine is an inactivated vaccine composed of capsular polysaccharide fragments of Neisseria meningitidis. There are four polysaccharide serotypes represented in the vaccine A. C. Y, and W-135. The type A polysaccharide consists of a polymer of Af-acctyl-O-mannosaminc phosphate the group C polysaccharide is mostly Af-acctyl-O-ace-tylncuraminic acid. Indications are... 

Two sulfone-based nucleoside diphosphate isosters (71a,b) has been synthesised and reported by Gervay-Hague to be inhibitors of Neisseria meningitidis CMP-sialic acid synthetase, which is a key enzyme in the biosynthesis of capsular polysaccharides required for bacterial infection. The synthetic methodology includes a condensation reaction of the nucleoside aldehydes with bisphosphonate Horner-Wadsworth-Emmons reagents (Scheme 4). The deprotection sequence was crucial for the appropriate completion of the synthetic targets. 

Selander B, Kayhty H, Wtdege E et al. Vaccination responses to capsular polysaccharides of Neisseria meningitidis and liaemophilus influenzae Type b in two C2-deficient sisters alternative pathway-mediated bacterial killing and evidence for a novel type of blocking IgG. J Clin Immunol 2000 20 138-149. 

The trichloroacetlmldate (2) has been used in the synthesis of the repeating unit of the capsular polysaccharide of Neisseria meningitidis [0-6-D-GlcNAc-( 1 3)-0-B-D-GlcNAc-( 1 -> 3)-0-3-D-... 

The capsular polysaccharide antigen of Neisseria meningitidis serogroup 29-e, composed of equimolar amounts of 2-acetamido-2-deoxy-D-galactose and 3-deoxy-D-wawio-octulosonic acid (KDO), has been identified, using a combination of chemical and n.m.r. spectroscopic techniques, as a linear polysaccharide having alternative sequences of the two residues (16). ... 

Deoxy-D-mfmw-octulosonic acid has been identified as the principal component (53%) of the capsular polysaccharide antigen of Neisseria meningitidis serogroup 29-e. ... 

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