V-Acetyl neuraminic acid

Bossart-Whitaker P, Carson M, Babu YS, Smith CD, Laver WG, Air GM (1993) Three-dimensional structure of influenza A N9 neuraminidase and its complex with the inhibitor 2-deoxy 2,3-dehydro-V-acetyl neuraminic acid. J Mol Biol 232 1069-1083 Burmeister WP, Ruigrok RW, Cusack S (1992) The 2.2 A resolution crystal structure of influenza... 

Mahmoudian, M., Noble, D., Drake, C.S. etal. (1997) An efficient process for production of N-acetylneuraminic acid using V-acetyl neuraminic acid aldolase. Enzyme and Microbial Technology, 20 (5), 393—400. 

There also seemed to be no requirement for glycosylation of interferon for secretion, although a partial involvement cannot be strictly excluded. Interferon formed in the presence of glycosylation inhibitors showed less charge-heterogeneity, which is caused by. V-acetyl-neuraminic acid in the native molecules.333 534... 

The structures of TV-acetyl neuraminic acid (sialic acid Neu5Ac) and the 2-deoxy-2,3-dehydro-/V-acetyl neuraminic acid (Neu5Ac2en) inhibitor complexed with N2 neuraminidase [66] revealed the nature of the interactions of the... 

Fraction Galactose N-acetyl- galactosamine. V-acetyl- neuraminic acid Phosphate... 

When CMP-iV-acetyl neuraminic acid synthetase (EC 2.7.7.43) of Haemophilus influenzae, polyphosphate kinase and CMP kinase were added to the reaction mixture containing equimolar concentrations (15 mM) of CMP and V-acetyl neuraminic acid, and PolyP (150 mM in terms of phosphate), CMP-iY-acetyl neuraminic acid was synthesized up to 67 % yield (Ishige et al, 2001). 

A substance later shown to be muramic acid (see Section IV,6) was clearly different from the product of Vi antigen, and, although the strain used in some of the Vi studies (Escherichia coli 5396/38) is one in which sialic acid has been detected, the properties of the Vi antigen are markedly different from the neuraminic acid pol3oner. Infrared spectra recorded for crystalline V-acetyl-neuraminic acid exhibit full detail and cannot be compared with that published for aminohexuronic acid, but that of the amorphous neuraminic acid polymer (colominic acid) is rather similar to that of the Vi antigen polymeric unit, since their functional groups are very similar. The relationship of these substances to the property of 0 -inagglutinability is discussed later (see p. 336). 

When the fraction soluble in 90 %-saturated ammonium sulfate (the fraction containing colominic acid) was recovered by dialysis instead of by precipitation, some very interesting products, having lower molecular weight and containing neuraminic acid, were found. It was confirmed that colominic acid contained V-acetyl-neuraminic acid to the extent of at least 80 %. 

U. Kragl, D. Gygax, O. Ghrsalba, and C. Wandrey, Enzymatic two-step synthesis of V-acetyl-neuraminic acid in the enzyme membrane reactor, Angew. Chem. Int. Ed. Engl. 30 827 (1991). M.-J. Kim, W. J. Hennen, H. M. Sweers, and C.-H. Wong, Enzymes in carbohydrate synthesis W-Acetylneuraminic acid aldolase catalyzed reactions and preparation of 7V-acetyl-2-deoxy-D-neuraminic acid derivatives, J. Am. Chem. Soc. 770 6481 (1988). 

V-acetyl neuraminic acid (sialic acid) nuclear magnetic resonance nuclear Overhauser effect phenyl... 

CHEMISTRY AND ANTIVIRAL ACTIVITY Zanamivir (4-guanidino-2,4-dideoxy-2, 3-dehydro-/V-acetyl neuraminic acid) is a siaMc acid analog that potently and specifically inhibits the neuraminidases of influenza A and B viruses. Depending on the strain, zanamivir competitively inhibits influenza neuraminidase activity but affects neuraminidases from other pathogens and mammalian sources only at much higher concentrations. Zanamivir inhibits in vitro replication of influenza A and B viruses, including amantadine- and rimantadine-resistant strains and several oseltamivir-resistant variants. 

Sialyltransferases catalyze the transfer of V-acetyl-neuraminic acid from CMP-NeuSAc to acceptors at or near the nonreducing terminus of oligosaccharide chains of glycoproteins or glycolipids [69]. Cell-surface sialic acid residues are important as antigenic determinants, as ligands for cellular adhesion and receptors for the bind-... 

Stereodivergent synthesis of 4S- and 4/ -configured 6-dialkylaminocarbonyl /V-acetyl-neuraminic acid derivatives (53 and 54), catalyzed by NeuA E192N/T167G (NeuA =)... 

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