Necropsy, animal

Inject 3H-Thymidine Necropsy animals 5 hr later, remove and process auricular lymph nodes. 

Signs of acute endosulfan intoxication similar to those reported in humans have been observed in animals. Also, cerebral congestion and edema is often observed at necropsy in animals that die following acute ingestion of endosulfan (Boyd and Dobos 1969 Boyd et al. 1970 Terziev et al. 1974). The severe... 

There is no evidence from animal studies that diisopropyl methylphosphonate directly affects the cardiovascular system except at very high doses (Palmer et al. 1979). No anomalous necropsy or histopathological effects were noted in rats that received diisopropyl methylphosphonate in the diet at doses of 0, 30, 100, or 300 mg/kg/day for 90 days (Hart 1976). Similarly, no abnormalities were noted in mice that received the compound in the diet at doses of 0, 27, 91, or 273 mg/kg/day for 90 days (Hart... 

Oral ingestion of diisopropyl methylphosphonate does not appear to induce respiratory effects. Necropsy of both male and female rats that died as the result of a single dose (928, 1362, or 2,000 mg/kg) of diisopropyl methylphosphonate administered by gastric intubation revealed some hyperemia of the lungs however, most animals displayed no abnormalities (Hart 1976). No abnormal necropsy findings were noted in Swiss Webster mice dosed similarly in a companion study (Hart 1976). No important abnormalities were noted in the necropsy of rats receiving diisopropyl methylphosphonate in the diet (0,... 

Kitten, 3 months old, died in 6 h following hypersalivation, ataxia, depression, and seizures. No histopathology at necropsy brain AChE activity normal. Fenvalerate residues, in pg/kg, were 345,000 in skin, 230 in kidney, 150 in liver, 10 in brain. Adult (4-year-old) showed signs of toxicosis 4 h after topical application by 30 h, animal had lowered body temperature, bradycardia, and other signs of fenvalerate poisoning. At death, shortly thereafter, fenvalerate residues were 1000 pg/kg in skin and 20 pg/kg in liver... 

Keep in mind that the objective of such studies is to gain information about lethality and gross tolerance. For nonrodents (especially monkeys), if none of the animals dies or demonstrates obvious signs of toxicity, little would be gained by euthanizing and necropsying such animals. They can be saved and used again,... 

Daily checks for mortality and morbidity, gross necropsy on animals found dead. 

Studies are often one to two weeks in duration and routinely include body weight determinations, clinical observations, and gross necropsy findings. Additional antemortem studies may be performed as appropriate, especially in large animals (e.g., observation of local reactogenicity, pharmacokinetic evaluations, hematological and/or clinical biochemistry measurements). Histological evaluations may also be performed. 

Selecting a subset of animals or test systems from a study to make some measurement (which either destroys or stresses the measured system, or is expensive) at an interval during a study. This may include such cases as doing interim necropsies in a chronic study or collecting and analyzing blood samples from some animals during a subchronic study. 

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