Gross necropsy

A 50-year-old white male died after intentional methyl parathion ingestion (Wofatox liquid) (Fazekas and Rengei 1964). The estimated dose of methyl parathion was 1,840 mg. Gross necropsy findings consisted... 

HCDD. Administration of 0.1-100 / g HCDD/kg/day was associated with a dose-related decrease in maternal weight-gain during gestation. Gross necropsy examination at the time of cesarean section revealed evidence of maternal toxicity only among dams receiving 100 /xg/kg/day (pale, friable liver 3/20 dams serous atrophy of fat, 1/20 dams). 

Testicular atrophy was observed upon gross necropsy after male rats were continuously exposed for 90 days to an aerosol concentration of 101 mg/m3 Durad MP280, but not in rats exposed to 10.3 mg/m3 (MacEwen and Vemot 1983). Testicular atrophy was not observed in male rats continuously exposed for 90 days to an aerosol concentration of 100 mg/m3 Fyrquel 220 (MacEwen and Vemot 1983). 

Daily checks for mortality and morbidity, gross necropsy on animals found dead. 

Interim sacrifice, gross necropsies and organ collection, clin lab, 5/sex/dosage... 

TABLE 5.9. Examples of Gross Necropsy Findings from Acute Toxicity Studies... 

Studies are often one to two weeks in duration and routinely include body weight determinations, clinical observations, and gross necropsy findings. Additional antemortem studies may be performed as appropriate, especially in large animals (e.g., observation of local reactogenicity, pharmacokinetic evaluations, hematological and/or clinical biochemistry measurements). Histological evaluations may also be performed. 

Deaths occurred in pregnant female Crl CD rats and CD mice exposed to 1,2-dibromoethane for 23 hours per day over a 10-day period. Female rats and mice had increased mortality when exposed to 80 ppm of 1,2-dibromoethane while female mice also had significant mortality when exposed to concentrations of 38 ppm 1,2-dibromoethane (Short et al. 1978). Twenty percent mortality occurred in female Crl CD rats exposed to 80 ppm 1,2-dibromoethane over a 3-week period mortality did not occur at lower concentrations of 20 or 39 ppm. Male rats exposed to 89 ppm 1,2-dibromoethane over a 10-week period had 21% mortality but mortality did not occur at lower concentrations of 19 or 39 ppm (Short et al. 1979). There was no gross necropsy or histopathologic examination to establish the cause of death as related to chemical toxicity in either of these studies, which were focused primarily on development and reproduction. 

Gross necropsy (full, detailed, all adult animals)... 

Detailed clinical observations Body weight and food/water consumption Gross necropsy (moribund animals, animals that die during the study)... 

Pregnancy length and birth Fetal and pup growth and survival until day 4 Gross necropsy (adult animals, special attention to reproductive organs)... 

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