NCBI databases Biotechnology Information

Sequences of the genes/cDNAs can be retrieved from databases on the Internet at various web sites. For example, GeneBank (at the National Center for Biotechnology Information, NCBI) is at http //www.ncbi.nlm.nih.gov/ Web/Search/index.html. The EMBL Nucleotide Sequence database (through the European Bioinformatic Institute, EBI) can be found at http //www.ebi.ac.uk/queries/queries.html, whilst that of the DNA Data Bank of Japan is at http //www.ddbj.nig.ac.jp/. 

The PepSeq program of Micromass s ProteinLynx software package was used for de novo analysis of the sequence (MS/MS) data. MS-Pattern of ProteinProspectror55 used the sequence tag determined from PepSeq to search the nonredundant database of the National Center for Biotechnology Information (NCBI) for protein identification. 

Some Internet search sites that I have found particularly useful include the National Center for Biotechnology information (NCBI) Entrez cross-database search page (http //www.ncbi. nlm.nih.gov/sites/gquery), which includes PubMed Central containing a limited number of free, full text journal articles. In addition, High Wire Press run by Stanford University also contains many free articles from established journals (http //highwire.stanford.edu/) and is able to search the PubMed database simultaneously. 

GeneBank (http //www.ncbi.nlm.nih.gov/Genbank/) database directed by the National Center of Biotechnological Information. 

PubMed was developed by the National Center for Biotechnology Information (NCBI) at the National Library of Medicine (NLM) at the National Institutes of Health (NIH). The PubMed database was developed in conjunction with publishers of biomedical literature as a search tool for accessing literature citations and linking to full-text journal articles at Web sites of participating publishers. Publishers that participate in PubMed supply NLM with their citations electronically prior to or at the time of publication. 

ExPASy (Expert Protein Analysis System, www.expasy.ch) or the National Centre for Biotechnology Information (NCBI, www.ncbi.nlm.gov) websites. Both websites provide bioinformatics tools, links to sequence databases and extensive bibliographic resources. As an example of the wealth of information available on individual enzymes, at the time of writing a search based on nitrilase in the Entrez protein section of NCBI will recover more than 10000 references to nitrilase enzyme amino acid sequences. These can be rapidly screened online by organism, and the individual entries will have links to amino acid and gene sequence, relevant literature and information on protein features (such as conserved domains). 

Databases at the EBI for nucleotide/protein searches (data largely overlap that at National Center for Biotechnology Information (NCBI)). 

The National Center for Biotechnology Information (NCBI). Located at the National Library of Medicine in Bethesda, MD, USA. The home of the GenBank DNA sequence database PubMed literature search engine sequence search tools (e.g., PSI-BLAST) genomic sequence navigation tools. A substantial repository of resources in all areas of bioinformatics. 

OMlM stands for Online MendeUan Inheritance in Man. It is a World Wide Web database (http //www.ncbi.nlm.nih.gov/omim) developed under the auspices of the National Center for Biotechnology Information (NCBI). It consists of a catalog of human genes and genetic disorders and contains textual information, pictures, and reference information. It also contains links to NCBFs Entre/database of MEDLINE articles and sequence information. Individual disorders may be found by entering the OMIM numbers into the search engine of the database. 

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