Nalgene

A Nalgene 15-500 polyethylene bottle (Nalge Co., Rochester, New York) was used. The side arm coming off the shoulder of the bottle is kept well above the liquid level, the constricted tip removed, and the end of the tube connected to a drying tube containing clay plate chips impregnated with concentrated sulfuric acid. 

DuPont/Sorvall RC-5B refrigerated centrifuge Nalgene centrifuge bottle, 250-mL... 

Electronic analytical balance Volumetric flasks 100- to 1000-mL Volumetric glass pipets, various sizes Pasteur pipets, 5.75- and 9-in lengths Refrigerated centrifuge Nalgene centrifuge bottle, 250-mL Disposable syringes, 5-cm ... 

Cuculic and Branica [788] used differential pulse ASV to study the adsorption of cadmium, lead, and copper on glass, quartz, and Nalgene sample containers. Nalgene was shown to be the best for sample storage, and quartz the best for electroanalytical vessels. 

Cuculic and Branica [788] applied differential pulse anodic stripping voltammetry to a study of the adsorption of cadmium, copper, and lead in seawater onto electrochemical glass vessels, quartz cells, and Nalgene sample bottles. Nalgene was best for sample storage and quartz was best for electroanalytical vessels. 

Filter the eluted RNA solution through a 0.22 pirn sterile filtration unit (Nalgene) to remove the acrylamide particles. 

Procedure. Weigh 10 g air-dry soil, sieved to 2 mm (10 mesh) using a stainless steel sieve into a 175-ml square HDPE (e.g. Nalgene) plastic screw-cap... 

In order to understand the behavior of the soil-like aerosols, samples of exposed surface soils were collected in the vicinity of each of the sites. Additional samples were obtained from locations in the Ute Indian area, for a total of 110 soil samples. The samples were collected by first selecting a location representative of the surrounding area. Factors considered at each site Included ground cover, agricultural practices, and a judgement of the ability of the surface to be resuspended. An area approximately 15 cm square was removed to a depth of 2-3 cm and transported to Davis in a plastic zip-lock bag or a nalgene bottle. 

IVPM (incomplete) For each 100 ml of medium needed, the following components are mixed and the pH adjusted to 7.0-7.2 using IN NaOH or IN HC1 as needed. The medium is then filter sterilized using a 0.2-pm Nalgene filter flask, which causes an increase of the final pH by approximately 0.18 ... 

Sample Collection. Pelagic lake-water samples were collected at Crystal Lake, an engineered mesotrophic lake in Champaign County, Illinois. It has an average depth of 10 feet with a maximum depth of 13 feet and is fed by ground-water from a 200-foot-deep well. For each sample 250-500 L of water was filtered with a plankton net and stored in 55-L polyethylene (Nalgene) containers until processed in the laboratory approximately 30 min later. 

Centrifuge with eight-cell rotor to hold 50-ml polycarbonate centrifuge tubes (e.g., Sorvall SS-34 rotor and Nalgene tubes)... 

Some of the fibers in each batch were aged in humid air, in deionized (DI) water, or in silane solution. All of the aging was carried out at 35°C for 30 days. A commercial (Blue-M) controlled atmosphere chamber held the relative humidity (RH) at 70% and the temperature at 34°C. The silane solutions were prepared by adding the requisite amount (1% by volume) of unhydrolyzed y-APS (A-1100 Union Carbide) to triply distilled water or to acetic acid solution. A hydrolysis time of approximately 8 h was allowed prior to adding fibers to the solution. This yielded solutions at pH 10 and pH 4, respectively. The fibers were suspended in Nalgene containers, where the glass surface area-to-solution volume ratio was fixed at 50 cm1. In all cases, the fibers were dried in air at 75°C and the solutions were analyzed for their Si, Al, Ca, and B contents. Table 1 presents the results of these solution analyses. 

Sterile trace element free 100 ml Nalgene bottles (for ICP-MS and ICP-AES analysis)... 

To avoid cell damage as a result of the rapid formation of ice crystals a freezing rate of 1°C per minute is optimal. If Nalgene tubs are not available, vials can be wrapped loosely in cotton wool to achieve a similar effect, but this has a more variable success rate. 

Cryo 1°C freezing containers (cat. no. 5100 Nalgene, Nalge Europe Ltd, Rotherwas, Hereford, UK). 

Place the vials were placed in a Nalgene freezing tub filled with isopropanol then place in a -70°C freezer overnight (see Note 24). 

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