Nalgene container

Sample Collection. Pelagic lake-water samples were collected at Crystal Lake, an engineered mesotrophic lake in Champaign County, Illinois. It has an average depth of 10 feet with a maximum depth of 13 feet and is fed by ground-water from a 200-foot-deep well. For each sample 250-500 L of water was filtered with a plankton net and stored in 55-L polyethylene (Nalgene) containers until processed in the laboratory approximately 30 min later. 

Some of the fibers in each batch were aged in humid air, in deionized (DI) water, or in silane solution. All of the aging was carried out at 35°C for 30 days. A commercial (Blue-M) controlled atmosphere chamber held the relative humidity (RH) at 70% and the temperature at 34°C. The silane solutions were prepared by adding the requisite amount (1% by volume) of unhydrolyzed y-APS (A-1100 Union Carbide) to triply distilled water or to acetic acid solution. A hydrolysis time of approximately 8 h was allowed prior to adding fibers to the solution. This yielded solutions at pH 10 and pH 4, respectively. The fibers were suspended in Nalgene containers, where the glass surface area-to-solution volume ratio was fixed at 50 cm1. In all cases, the fibers were dried in air at 75°C and the solutions were analyzed for their Si, Al, Ca, and B contents. Table 1 presents the results of these solution analyses. 

The floor under the angiography machine must be covered with a drape protecting against potential leakage or other contaminations to the floor, and for radioprotection an acrylic Nalgene shield and a Nalgene container are needed to store contaminated material during and after the delivery of °Y microspheres, e.g., catheters and drapes. 

A Nalgene 15-500 polyethylene bottle (Nalge Co., Rochester, New York) was used. The side arm coming off the shoulder of the bottle is kept well above the liquid level, the constricted tip removed, and the end of the tube connected to a drying tube containing clay plate chips impregnated with concentrated sulfuric acid. 

Cuculic and Branica [788] used differential pulse ASV to study the adsorption of cadmium, lead, and copper on glass, quartz, and Nalgene sample containers. Nalgene was shown to be the best for sample storage, and quartz the best for electroanalytical vessels. 

Cryo 1°C freezing containers (cat. no. 5100 Nalgene, Nalge Europe Ltd, Rotherwas, Hereford, UK). 

Nalgene (8000) General laboratory use, good flexibility, nonflammable, clear. Not good with organic solvents and most oils, OK with weak acids, but best to avoid strong acids and alkalis, contains plasticizers that can leach out during operations such as distillation. 

Polyethylene Nalgene 8010 Economical, good general chemical resistance, contains no plasticizers. Not autoclavable, very stiff, translucent, flammable. 

Polyurethane (poly- ester/polyether urethane) Hygenic Corp. (HC480ARJ Nalgene 8030 Contains no plasticizers. Can be used both for vacuum or pressure systems. Has higher chemical resistance to fuels, oils, and some solvents than does PVC tubing. Not autoclavable, stiffer than PVC, flammable, not recommended with strong acids or alkalis. 

Solvent wash bottles. The Nalgene 500-ml. polyethylene wash bottles are recommended as containers for common solvents (see Fig. S-4). The name, symbol, or formula of a solvent can be written on a bottle with a Magic Marker or wax pencil. A volume up to 25 ml. per squeeze can be delivered from the spout into a graduate a larger volume can be poured out of the central opening. For crystallizations and for the quick cleaning of apparatus it is convenient to have available one bottle for each frequently used solvent.-... 

Preparation,a [1, 135, at end]. Since a glass surface catalyzes the decomposition of the peracid, the reaction of m-chlorobenzoyl chloride with hydrogen peroxide in the presence of magnesium sulfate heptahydrate, aqueous sodium hydroxide, and dioxane is carried out in a polyethylene beaker (Nalgene). The product obtained contains 80-85% active oxygen. 

To freeze cells, pellet cells by spinning at 100 x for 10 min. Aspirate medium and resuspend in chilled culture medium plus 10% DMSO at 10 million cells/ml. Aliquot 1.8 ml each into cryovials, place in Nalgene cryofreezing container with isopropanol at -80°C for 2 days, and then transfer to liquid nitrogen storage (tee Note 10). 

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