Soil isolate

Strains of Streptomyces isolated from a range of nrban, agricnltnral, and forest soils isolated on a standard medinm displayed resistance to antibiotics that were well established in clinical practice. Resistance was fonnd among all classes of antibiotics, and a distnrbing nnmber of isolates were resistant to between 5 and 10 of them (D Costa et al. 2006). 

Further experience has demonstrated that it was not solely the choice of assay but the great good fortune to have received a group of cultures from the Kitasato Institute and to have made the decision to screen these cultures in the dubius assay. One of these cultures, OS-3153, was active. The screening of several tens of thousands of soil isolates in this assay has failed to detect any remotely similar anthelmintic activity. Of the fermentation products discussed, this is the only one where the activity for which the product was eventually marketed was found by direct screening. (Zeranol might be considered to be another example, but it was not discovered by screening.)... 

In order to obtain rapid metabolism and rapid heat generation from aromatic compounds related to lignin, several soil isolates were used. P. putida ATCC 11172 did not grow easily on compounds such as syringic acid and certain other compounds more lignin-like. However, soils collected locally yielded bacteria, first from enrichment cultures and then grown on plates or defined liquid culture, were able to combust the compounds listed in Table... 

Most agriculturally based pollutants probably return to the environment at points that are difficult to identify and are called nonpoint source emissions. Pesticides leave farms as runoff or leach through the soil. Isolating and treating them is not often possible. While techniques such as ditching and basins are being adopted, the problem of treatment in the dilute state in which pollutants will be isolated is a serious concern. 

A low cobalt concentration was shown by Hendlin and Ruger (1950) to limit synthesis of vitamin Bi2. Cobalt comprises about 4% of the molecule. Working with 13 cultures, including a strain of Streptomyces griseus, unidentified rumen and soil isolates, a strain of Mycobacterium smegmatis, and Pseudomonas species, Hendlin and Ruger (1950) found that addition of 1 to 2 ppm of cobalt increased yield by threefold. 

Levy-Schil, S. Soubrier, F., Crutz-LeCoq, A.M., et al. 1995. Aliphatic nitrilase from a soil isolated Comamonas testosteroni sp. Gene cloning and overexpression, purification and primary structure. Gene, 161 15-20. 

Soil isolates produce natural products, most of which have physiological properties. 

Nakagawa, K., Sato, K., Okazaki, T., and Torikata, A. (1992) Microbial conversion of milbemycins 13p,29-Dihydroxylation of milbemycins by soil isolate Streptomyces cavourensis. J. Antibiot. 44, 803-805. 

The hint, which led us to this finding, was the accidental observation of paralytic syndrome in silkworms that had digested an extract of a certain isolate. We had been screening numerous soil isolates for their insecticidal activity against silkworms upon oral administration in the usual manner. It was of great interest that the strain Aspergillus japonicus Saito JV-23 resulted in paralysis in silkworms. 

FH Osborne, HE Ehrlich. Oxidation of arsenite by a soil isolate of Alcaligenes. J Appl Bacteriol 41 295-305, 1976. 

Polyesters Poly(ethylene adipate) (Mn) = 3000) Polycaprolactone (M = 25,000) Biomass and analysis Biomass and TOC Penicillium sp. (soil isolate) [107]... 

Nelson, I.M. Biologically Induced Hydrolysis of Parathion in Soil Isolation of Hydrolyzing Bacteria, Soil Biol. Biochem., 14 219-222 (1982). 

Various research projects in areas of structural and soil isolation, dynamic interface properties of geosynthetics, seismic permanent deformations, and liquefaction mitigation have been conducted using this facility. A recent research project conducted on liquefaction mitigation will be presented in the following section. 

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