Mutagenicity tests Ames test

Salmonella Mutagenicity Test (Ames Test). The methods of bacterial culture, the verification of genetic markers, and the plate incorporation assay were essentially the same as described previously (14, 15). Petri dishes (90 mm) containing about 20 mL of 1.2 Noble agar in minimal Vogel Bonner Medium E supplied with excess biotine and... 

FMC. 1992b. Durad 550B. Salmonella/mammalian-microsome plate incorporation mutagenicity assay (Ames Test). Study No. 191-1222. FMC Corporation, Philadelphia, PA. 

Williams, L. R. Preston, J. E. Interim Procedures for Conducting the Salmonella-Microsomal Mutagenicity Assay (Ames Test) Environmental Monitoring Systems Laboratory Las Vegas, NV, 1983 EPA-600/4-82-068. 

Barbee GC, Brown KW, Thomas JC, et al. 1996. Mutagenic activity (Ames test) of wood-preserving waste sludge applied to soil. Bull Environ Contain Toxicol 57 54-62. 

The Salmonellajmtcvo ome mutagenicity assay (Ames test) is usually used to predict mutagenicity... 

IPBC is not a primary skin irritant, but is irritant and corrosive to the eyes. No skin sensitization effects were observed in the guinea pig maximization test. Ames test and micronucleus assay did not show signs of mutagenicity. 

Toxicology Acute oral toxicity LD50 (oral, rat) > 5.000 mg/kg no mutagenic activity (Ames Test) Eye contact may produce an oil Aim over the eye-ball causing a harmless reversible short lasting dimness of sight. 

Physicochemical properties requked include melting/boiling point, vapor pressure, solubiUty, and flammabiUty/explosion characteristics. The toxicological studies include acute toxicity tests, oral, inhalation, and dermal skin and eye kritation skin sensiti2ation subacute toxicity, oral, inhalation, and dermal and mutagenicity tests. In vitro reverse mutation assay (Ames test) on Salmonella typhimurium and/or E.scherichia coli and mammalian cytogenic test. In vivo mouse micronucleus test. 

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