Vogel-Bonner medium

Salmonella Mutagenicity Test (Ames Test). The methods of bacterial culture, the verification of genetic markers, and the plate incorporation assay were essentially the same as described previously (14, 15). Petri dishes (90 mm) containing about 20 mL of 1.2 Noble agar in minimal Vogel Bonner Medium E supplied with excess biotine and... 

Fig. 17. Effect of DEAE-dextran on the growth of E, coli. E. coli MRE 600 was grown in Vogel-Bonner medium to a density of 3-5 X 10 cells/ml. DEAE-dextran was added to final concentrations as indicated below and incubation continued at 37° C. At 0 time...

Mix and pour onto dried Vogel and Bonner minimal medium plates as in an Ames test, including an untreated control and a solvent control, if necessary. The final concentrations of test compound will be 5000, 1500, 500, 150 and 50 pg... 

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