Noble agar

Salmonella Mutagenicity Test (Ames Test). The methods of bacterial culture, the verification of genetic markers, and the plate incorporation assay were essentially the same as described previously (14, 15). Petri dishes (90 mm) containing about 20 mL of 1.2 Noble agar in minimal Vogel Bonner Medium E supplied with excess biotine and... 

To make up 2.5% Noble agar (Difco Labs.) add 25 g agar powder to 500 ml distilled water and make up to 1 1. Place the flask in a container of boiling water until the agar dissolves. Dispense 25 ml amounts into universal containers with metal caps and autoclave at 15 lb pressure for 15 min. Store at room temperature. 

Adjust to pH 7.4 and add 10 g of Difco Noble Agar if a basic agar is required. 

NaN03, 1.0 g and 1.0 ml of trace salts solution. This consisted of, per milliliter NaCl, 0.005 g FeSO -THgO, 0.002 g ZnS0it-7H20, 0.002 g and MnSO -HpO, 0.0007 g. The pH was adjusted to 6.5 with 10% (w/v) NaOH. For solid media, BMS was supplemented with 10.0 g of Noble Agar (Difco, Detroit, Ml) per liter. For experiments with nitrogen-free medium, both NH Cl and NaNO were omitted, NaCl, 1.0 g/1. was substituted. 

Sterol Content Tween 80 Soybean Lecithin (ICN) Soybean Lecithin (Sigma) Noble Agar... 

Noble agar can be substituted for hydrophobic agar used in the original protocol (IS). 

Penicillium funiculosum (ATCC 9644), obtained from National Regional Research Labs, Peoria, 111., was maintained in slant tubes containing a basal mineral salts media in noble agar with 0.1% PHB. A slant tube of the culture was used to inoculate a Bio-Flo fermienter containing 200 mLs of a basal mineral salts broth (pR 6.5) with 0.1% PHB as the sole carbon... 

Dissolve agar (Agar Noble, Difco Laboratories) in 50 mM PB, pH 7.3, containing 150 mM NaCI in an electric oven. Spread the gel on a slide glass. Make a 3-mm diameter well in the center of the gel, and 6 wells around the center well at a distance of 7.5 mm from center to center of the wells. Add 10 [il of antigen (100. ig/ml) to the central well and 10 [il of 2-fold serially diluted serum to the outer wells. Incubate the gel at 4 °C for 1-2 days in a humid atmosphere in a Petri dish. Read the maximum dilution that results in an immunoprecipitin line. Usually, the antibody titer (the dilution factor) of rabbit serum immunized with component II is higher than that for component I. 

Affi-Gel 15 Agar Noble CM-Sepahdex A-50 Cooked meat medium DEAE-Sepahdex A-50 Ficoll 400 Hydroxyapatite Nutrient broth Sephacryl S-300 TRITC-anti rabbit IgG... 

U.S.A. is very useful other products of Difco— Special Agar Noble and Difco Purified Agar —come close to maximum purification. The product of Merck A. G., Darmstadt, Germany (Agar-agar, pulv. subst.)... 

The amount of Gelrite is adjusted to give soft plant tissue culture media, and in our hands, media can be stored and remelted once before use. A slightly different autoclave cycle may, however, produce very sloppy media that will not set. Increase Gelrite concentration or use agar noble instead. 

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