Multiple sclerosis model experimental allergic

The role of ac tivated microglia in neuronal survival during inflammatory conditions has been extensively studied in the models of experimental allergic/autoimmune encephalomyelitis (EAE) and in the animal model of Multiple Sclerosis (MS) (Antel and Owens, 1999 Becher etal., 2000 Biemacki etal., 2005 Jack et al., 2005). A rapid recruitment of blood-borne monocytes, an... 

Siiram S, Steiner I (2005) Experimental allergic encephalomyelitis A misleading model of multiple sclerosis. Ann Neurol 58 939—945. 

EAE experimental allergic/autoimmune encephalitis is an animal model for demyelinating diseases, such as multiple sclerosis. It is induced by injection of myelin basic protein or whole CNS tissue together with adjuvants. 

For some studies in the CNS, e.g. for research on the role of macrophages in experimental allergic encephalomyelitis (EAE), a rodent model for multiple sclerosis (MS), clodronate liposomes should be mannosylated (29, 30). 

Multiple sclerosis (MS) has traditionally been viewed as a disorder in which myelin is the primary target. However, there is recent evidence for abnormal SNS expression in experimental models of demyelination and in MS. Black et al (1999b) studied Na channel expression in the taiep rat, a mutant model in which myelin is initially formed normally, but then lost as a result of an oligodendrocyte abnormality. They observed the abnormal expression of SNS Na channel mRNA and protein in Purkinje cells following loss of myelin. More recently. Black et al (2000) demonstrated that SNS mRNA and protein, which are not detectable in normal Purkinje cells, are expressed within Purkinje cells in a mouse model of MS, chronic relapsing experimental allergic encephalomyelitis. Black et al (2000) have also demonstrated the expression of SNS mRNA (Fig. 7a, b) and protein (Fig. 7e, f) within cerebellar Purkinje cells from tissue obtained post-mortem from MS patients, but not in controls with no neurological disease (Fig. 7c, g). 

In mice with chronic relapsing experimental allergic encephalomyelitis (CRE AE, a model of multiple sclerosis), in the spinal cord (Baker et al. 2001)... 

Berrendero F, Sanchez A, Cabranes A, Puerta C, Ramos JA, Garcia-Merino A, Fernandez-Ruiz J (2001) Changes in cannabinoid CB(1) receptors in striatal and cortical regions of rats with experimental allergic encephalomyelitis, an animal model of multiple sclerosis. Synapse 41 195-202... 

In induced models, a susceptible animal strain is immunized with a mixture of an adjuvant and an autoantigen isolated from the target organ. Examples are adjuvant arthritis in the Lewis strain rat (Pearson, 1956) and experimental allergic encephalomyelitis, a model of multiple sclerosis (Ben-Nun Cohen, 1982). Induced models are often used to study the pathogenesis of and therapeutic venues for relevant autoimmune diseases. These models have been proposed as means to evaluate the immunomodulatory effects of chemicals on ongoing autoimmune diseases in a second tier of immunotoxicity testing. 

Experimental allergic encephalomyelitis (EAE). Autoimmune demyelinating disease induced in genetically susceptible mice, rats, or marmosets by immunization with myelin proteins or peptides. Animal model for multiple sclerosis. 

The MBPS are a family of proteins. Unlike PLP, MBPs are easily extracted from the membrane and are soluble in aqueous solution. The major MBP has no tertiary structure and has a molecular weight of 15,000 Daltons. MBP is located on the cytoplasmic face of myelin membranes. Antibodies directed against MBPs elicit experimental allergic encephalomyelitis (EAE), which has become a model system for understanding multiple sclerosis, a demyelinating disease. A model of how PLP and MBPs aid in stabilizing myelin is shown in Figure 48.14. 

It has been well established that a number of chemokines including, KC, IP-10, MIP-1 a, RANTES, MARC (murine MCP-3), and TCA-3 (murine 1-309) are upregulated during the course of murine experimental allergic encephalitis (EAE) a mouse model of multiple sclerosis (MS) (25). Glabinski et al. have also demonstrated that the chemokines... 

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