Mouse studies C57BL/6 strain

C57BL/6J is one of the most commonly used mouse strains for diabetes studies. Diet-induced obesity in this strain causes hyperglycemia, hyperinsulinemia, and the development of a fatty liver. One shortcoming of this model, however, is that diet alone is insufficient for the symptoms to progress to a later stage disease, such as beta-cell degeneration and diabetic nephropathy. 

C57BL/6 is the most commonly used strain in cardiovascular research so it is the most characterized with respect to cholesterol absorption and excretion. However, mouse strains do differ with respect to cholesterol absorption (41). Thus, it is very important to have accurate control groups for all absorption experiments. Mice should be matched for age, sex, genetic background, and even diet history (unless that is a parameter being studied). For example, while many investigators will purchase control mice from commercial suppliers or will select them from a wild-type colony or collection, the purist will use control mice from the... 

At present, we can induce CML in mice with high efficiency, shown by 100% induction of CML in mice (14). The same CML disease could be induced in most of the inbred mouse strain including C57BL/6, BALB/c, and viable gene knockout mice strains (15). Because all recipients develop CML with a short latency (about 3 weeks), this provides an excellent model for evaluating therapeutic agents for CML treatment (15). As CML is derived from the hematopoietic stem cells which harbor BCR-ABL oncogene, CML leukemia stem cells can also be studied in this model (15). In conclusion, this retroviral model system pro-... 

Here we describe a method for injecting lentivirus directly into specific regions of the mouse brain. This method is applicable to any viral-based approach used in studies of CNS function in the mouse. In addition, this method can be used in any mouse strain required for the specific behavioral end point being studied. For instance, in studies of cocaine or alcohol addiction, an investigator may choose to use the C57BL/6J strain because of its robust response to cocaine and propensity to drink large quantities of ethanol, whereas for other alcohol-related behaviors, the DBA/2J mouse might be chosen (7). 

Although precise occupational exposures were not known, most studies indicated that workers had been exposed to concentrations up to 250-300 ppm during at least part of a working day thus, 300 ppm was chosen. The C57BL/6 and CBA/Ca mouse strains were chosen for these studies because of their susceptibilities to ionizing radiation-induced thymic lymphoma. The strains are also recognized for their low spontaneous rates of AML, the disease most frequently associated with benzene exposure in humans. 

Over the next 30 years, two lines of evidence led to the identification of the AHR, the protein that functioned as the PAH sensor and regulated AHH activity. The first indications that such a receptor existed came from genetic studies of inbred mouse strains. Early studies demonstrated that C57BL/6 mice were much more responsive... 

These studies have identified quantitative variation of purine nucleoside phosphorylase which is heritable and stable within inbred mouse lines. Associated with the quantitative variability are electrophoretic differences between DBA/2J and C57BL/6J purine nucleoside phosphorylase and minor differences in thermal stability and affinity for nucleoside substrates. Additional studies are underway to characterize the gene products of these strains and map the Np-2 locus. 

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