Mouse myeloma cell line

In an attempt to overcome this problem Kohler and Milstein (1975, 1976) have reported the results of fusing a mouse myeloma cell line with spleen cells taken from a mouse immunised with sheep erythrocytes. The hybrids were cloned and clones secreting anti-sheep erythrocyte antibodies selected. 

Another approach towards human glyco-sylation is followed by the utilization of a humanized mouse cell line, a human/ mouse heterohybridoma presented by Dr. Volker Sandig from ProBioGen. I have known Volker for a couple of years, since when I invented a real-time PGR test kit for the detection of mycoplasma contamination in pharmaceutical products. Using an internal standard we developed this method for rapid in-process (IPG) control for production of biopharmaceuticals and ProBioGen was one of the partners participating in the validation of the system as they wanted to use it for rapid quality analysis of their designer cell lines. As we have learned from previous examples, the development of mammahan super-producer cells from CHO (or also from the mouse myeloma cell line, NSO) starter cell... 

Immunomodulatory, Immunosupressant. Blocks IL-2R alpha on surface of activated T lymphocytes. Produced by mouse myeloma cell line. Contains human heavy- and light-chain constant regions and murine heavy- and light-chain variable regions. 

NSO, non-lg secreting, non-L-chain synthesizing. 8-Azaguanine-resistant and HAT-sensitive mouse myeloma cell line... 

Kearney, J.F., Radbruch, A, Liesegang, B., and Rajewsky, K. (1979) A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell hues. /. Immunol. 123,1548-1550. 

Zauderer, M., Liberti, P., and Baglioni, C., 1973, Distribution of histone messenger RNA among free and membrane-associated polyribosomes of a mouse myeloma cell line, /. Mol. Biol. 79 577. 

Fig. 3. To produce monoclonal antibodies, antibody producing spleen cells troll] a mouse that has been immunized against an antigen am mixed with mouse myeloma cells. Under the proper conditions, pairs of the cells fuse to form antibody-producing hybrid-myeloma ( hybridoma ) cells, which can live indefinitely in culture. Individual hybridomas are grown in separate wells, and the antibodies they produce are tested against the antigen. When an effective cell line is identified, it is grown either in cuhure or in the body cavities of mice to produce large quantities of chemically identical, monoclonal antibodies (reprinted with permission from Olsen, 1986, p. 26, Copyright, National Academy Press)...

Cell Fusion Unlike antibody-secreting cells, myeloma cells, malignant tumor cells of the immune system, can be cultured continuously. Kohler and Milstein (1975) developed a method to fuse (hybridize) B-lymphocytes from the mouse spleen with mouse myeloma cells, so that the fused cell, hybrid-myeloma (or hybridoma) cell, can have the characteristic of the both cell lines that is, the production of specific antibodies and the immortality. Since the hybridoma is derived from a single B-lymphocyte, it produces only one kind of antibody, thus a monoclonal antibody. 

This is a mouse BALB/c myeloma cell line secreting IgA (Halpem Coffman, 1972 Lundblad et al, 1972 Oi et al, 1983). The cell line is used in transfection studies. 

The spleen cells are fused with mouse myeloma cells to become a continuous hybridoma cell line. A continuous hybridoma cell line with multiple B-cell clones produces many different antibody clones indicated by the different gray levels of... 

Apart from the concentrafion of sericin, its extraction method has been shown to influence cell viability, also. If compared to heat, acid, or alkaline extraction methods, urea-extracted silk sericin is linked to the lowest cell viability of L929 mouse fibroblast (Aramwit et al., 2010). Sericin can also be used to replace bovine semm in cell freezing media. It cryopreserves cells as effectively as standard freeze medium containing foetal bovine serum. This effect has been demonstrated for various cell lines including P3U1 myeloma cell line, Chinese hamster ovary CHO cells, human dermal fibroblasts, human epidermal keratinocytes, the rat phaeochromocytoma cell line PC 12 and Sf9 insect cells (Sasaki et al., 2005), human primary hepatocytes (Miyamoto et al., 2010), rat pancreatic... 

Exponentially growing mouse or rat myeloma cell line... 

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