Mouse antibody production tests

Mouse antibody production test (MAP) a broad screen test for known viruses potentially present in test article (both ceU lines). 

Mouse antibody production test (MAP) 434 Mouse, chimeric 657 Mouse embryonic fibroblast 286 Mouse, gene targeted 649 Mouse lymphage assay 1524 Mouse minute virus (MMV) 434 Mouse, transgenic 1111, 1162 6-MP 57... 

Acceptable bridging molecule systems have been developed which have also simplified the utilization of different detection systems. To illustrate this point, a researcher who has developed a unique monoclonal antibody (a primary antibody) in the mouse may select from a variety of commercially available products consisting of different detection systems (e.g. fluorescein, alkaline phosphatase, colloidal gold) attached to an immunoglobulin that will specifically bind to mouse antibodies (a secondary antibody). In this way the researcher may readily obtain and test a number of detection methods for visualizing target-probe interactions without having to directly label the monoclonal antibody probe. For nucleic acid probes, which in themselves are not readily immunodetectable, it is useful to incorporate or attach detectable moieties to the nucleotides. 

The —37 kDa band (36-38 kDa) is close in molecular mass to dysbindin-lB, which cannot be a degradation product of dysbindin-1 A because of its unique C-terminus (see Section 2.2.2.2.1). We cannot detect this band in the mouse brain with any of the dysbindin-1 antibodies we have tested, including one (UPenn 331) which has a high affinity for the —37 kDa band in human tissue. (UPenn 331 also recognizes the 50 kDa band in humans and mice, albeit with much less affinity, and hence can recognize both dysbindin-1 A and - IB, but not dysbindin-lC or other dysbindin paralogs as expected from the fact that it was raised against an aa sequence in the NTR of human dysbindin-lA and -IB absent in dysbindin-lC, -2 or -3.) In synaptosomes of the human brain, the —37 kDa isoform is heavily concentrated in synaptic vesicle fractions with much lesser amounts in the PSD fractions and very little in presynaptic membrane fractions (Talbot et al., submitted). 

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