Molecular cloning of genes

Gaud, C. and Lightowlers, M.W. (2003) Molecular cloning of genes encoding oncosphere proteins reveals conservation of modular protein structure in cestode antigens. Molecular and Biochemical Parasitology 127, 193-198. 

PJ Sykes, G Burns, J Menard, K Hatter, JR Sokatch. Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida. J Bacteriol 169 1619-1625, 1987. 

Morris, SM, Jr., Nilson, JH, Jenik, RA, Winberry, LK, McDevitt, MA, and Goodridge, AG, Molecular cloning of gene sequences for avian fatty acid synthase and evidence for nutritional regulation of fatty acid synthase mrna concentration, J Biol Chem, 1982. 257(6) 3225-3229. 

G.C. Barrdre, C.E. Barber, M.J. Daniels, Molecular cloning of genes i nvolved in the production of the extracellular polysaccharide xanthan by Xanthomonas campesiris pv. campestris. Inti. J. Biol. Macromol. 8 (1986) 372-374. 

The biochemistry of de novo PLP biosynthesis and molecular cloning of genes coding enzymes has been primarily investigated in the gram-negative bacterium Escherichia coli [2-8], Two distinct pathways for PLP de novo biosynthesis have been identified DXP (1-deoxy-o-xylulose 5-phosphate)-dependent and DXP-independent pathways. 

Cochran, B. H., Reffel, A. C., and Stiles, C. D. (1983) Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33, 939-947. 

Loros, J.J., Denome, S.A. and Dunlap, J.C. (1989) Molecular cloning of genes under control of the circadian clock. Neurospora Science, 243, 383-388. 

Jain, R.K. (1996) Xhe molecular cloning of genes specifying some enzymes of the 3,5-xylenol degradative pathway. Appl Microbiol Biotechnol, 45, 502-508. 

Pulciani S, Santos E, Lauver AV, et al. 1982b. Oncogenes in human tumor cell lines. Molecular cloning of a transforming gene from human bladder carcinoma cells. Proc Natl Acad Sci USA 79 2845-2849. 

Harriman,R.W.,Tieman,D.M and Handa,A.K (1991) Molecular cloning of tomato pectinmethylesterase gene and its expression in Rutgers, ripening inhibitor, non-ripening and never-ripe tomato finit. Plant Physiology. 97. 80-87. 

PelZ is a hydrophilic protein of 420 amino acids with a short hydrophobic sequence at its N-terminal end which has Ae characteristics of the signal sequences of exported proteins. The signal peptide may be 24 amino acids long, which would corroborate wiA the usual length encountered in prokaryotes. The molecular cloning of the pelZ gene in an expression vector pT7-6 allowed for the specific 35S-cysteine-methionine raAo-labelling of PelZ in E. coli K38. We could detect, in crude extracts, the presence of a precursor and a mature form of PelZ. After cell fractionation, Ae mature form of PelZ could be localized in Ae periplasm of E. coli. So PelZ appears to be a protein exported by Ae Sec-dependent system of translocation. 

Miura K, Y Tomioka, H Suzuki, M Yonezawa, T Hishinuma, M Mizugaki (1997) Molecular cloning of the nemA gene encoding A -ethylmaleimide reductase from Escherichia coli. Biol Pharm Bull 20 110-112. 

Goyal AK, GJ Zylstra (1996) Molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from Comamonas testosteroni. Appl Environ Microbiol 62 230-236. 

H. Murata, J. L. McEvoy, A. Chatterjee, A. Collmer, A. K. Chatterjee. Molecular cloning of an aepA gene that activates production of extracellular pectolytic, cellulolytic, and proteolytic enzymes in Erwinia carotovora subsp. carotovora. Mol. Plant Micr. Interact. 4 239 (1991). 

Sumi-Ichinose, C., Ichinose, H., Takahashi, E., Hori, T., and Nagatsu, T. (1992). Molecular-cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and serotonin biosynthesis. Biochemistry 31 2229-2238. 

Gantz, I., Schaffer, M., DelValle, J. et al. (1991). Molecular cloning of a gene encoding the histamine H2 receptor. Proc. Natl. Acad. Sci. USA 88, 429-33. 

Palva, I. (1982) Molecular cloning of alpha-amylase gene from Bacillus amyloliquefaciens and its expression in B. subtilis. Gene, 19 (1), 81-87. 

Connolly, B., Trenholme, K. and Smith, D.F. (1996) Molecular cloning of a myoD-like gene from the parasitic nematode, Trichinella spiralis. Molecular and Biochemical Parasitology 81, 137—149. 

Pihlajaniemi, T., Helaakoski, T., Tasanen, K., Myllyla, R., Huhtal, M.-L., Koivu, J.K. and Kivirikko, K.I. (1987) Molecular cloning of the 3-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBOJournals, 643-649. 

Zou, J. et al., Molecular cloning of the gene for interleukin-ip from Xenopus laevis and analysis of expression in vivo and in vitro, Immunogenetics, 51, 332, 2002. 

Phosphoinositase C (i.e. phosphoinositide-specific phospholipase C [PLC]) enzymes are found in the vast majority of mammalian cells. Molecular cloning of these enzymes, analysis of their predicted amino acid sequences and immunological cross-reactivity indicate that at least three major forms of the enzyme exist PLC-/I, -8 and -y. Each of these enzyme types is encoded by a distinct gene. More recent experiments using the polymerase chain reaction and molecular cloning have revealed even greater enzyme di-... 

Bowgogk, a. M., Molecular cloning of BRCAl a gene for early onset familial breast and ovarian cancer. Breast Cancer Res Treat, 1993, 28(2), 121—35. 

Tani, M., Okino, N., Moii, K., Tanigawa, T., Izu, H., and Ito, M., 2000b, Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. J. Biol. Chem. 275 11229-11234. 

Gantz I, Schaffer M, DelValle J Molecular cloning of a gene encoding the histamine Hj receptor. Proc Natl Acad Sci USA 1991 88 5937. 

Nicolas, R.H. and Goodwin, G.H. (1996) Molecular cloning of polybromo, a nuclear protein containing multiple domains including five bromodomains, a truncated HMG-box, and two repeats of a novel domain. Gene 175, 233-240. 

Choi, Y.C., Gu, W., Hecht, N.B., Feinberg, A.P., and Chae, C.B. (1996) Molecular cloning of mouse somatic and testis-specific H2B histone genes containing a methylated CpG island. DNA Cell Biol. 15, 495-504. 

Takahashi R, Sakuma A, Itoh K, et al Comparison of efficacy of lithium carbonate and chlorpromazine in mania report of collaborative study group on treatment of mania in Japan. Arch Gen Psychiatry 32 1310-1318, 1975 Takahashi Y, Kato K, Hayashizaki Y, et al Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine. Proc Natl Acad Sci U S A 82 1931-1935, 1985... 

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