Control slides

Perforated cylinder Conveying vanes on cone Discharge - control slide... 

Flow-control slide outomaticolly octuoted by height-controller pQddle... 

Figure 11-21D. Helical rotors refrigerant compressors. (1) Cutaway of a 100-ton intermediate compressor. The intermediate Helirotor compressor has only three moving parts the two rotor assemblies and the capacity controlling slide valve. The general purpose Helirotor compressor has only four moving parts two rotor assemblies, the variable unloader valve, and the step unloader valve. Unlike reciprocating compressors, the Trane Helirotor compressor has no pistons, connecting rods, suction and discharge valves, or mechanical oil pump.

In order to allow for more control during the evaporation, the solution can be placed in a motor-controlled sliding [ 50] or rolling apparatus [51] which uses capillary force to confine the solution between two glass surfaces. The motor determines the speed at which the solution edge is drawn over the substrate and is one of the main parameters to control patterning. Droplet, stripe and ladder patterns have been observed. 

Control slides should be used to monitor the staining. Specimens containing protozoa are best for controls however, feces containing inflammatory cells or added buffy-coat leukocytes also are satisfactory. 

Sompuram SR, Kodela V, Zhang K, et al. A novel quality control slide for quantitative immunohistochemistry testing. J. Histochem. Cytochem. 2002 50 1425-1434. 

Figure 6.9 The HER2 antigen/gene correctly demonstrated in the UK NEQAS cell line control slides (indicated top to bottom) stained with four commercially validated systems (running left to right) (a) Dako HercepTest , (b) Leica Microsystems Oracle HER2 Bond IHC System, (c) Ventana Medical Systems Pathway 4B5, and (d) Vysis PathYysion HER2 FISH. See color insert.

Figure 7.4 is a photograph of a portion of the peptide controls slide printer. A stack of microscope slides, ready for printing, is at the far left. The slides are automatically ejected from the stack, one at a time. The slides are moved on a conveyer to the right, positioning them under the print head. The print head has eight nozzles, out of which microliter-sized droplets are ejected onto an underlying slide. The slide conveyer then places a new slide under the nozzles, and the process repeats. 

Figure 10.4 Schematic concept for a process control slide. The number of rows of analytes printed onto the slide depend on the number of process steps which need to be monitored.

As a test of reproducibility of spot printing and staining, a 10 spot by 10 row array (100 spots total) were printed on three slides. All slides were stained in a single staining run, using the same secondary detection chemistry with DAB as a chromogen. While DAB cannot provide accurate photometric measurements, it can provide relative comparisons from one spot to another on the same slide. When the stained control slides were analyzed in this... 

Figure 10.5 A series of slides printed with dilutions of mouse serum and rabbit serum. There are five replicates of each species, and all slides have identical printing. The secondary antibody cocktail was constructed according to the labels on each slide. This demonstrates that a control slide can easily detect sensitivity changes in secondary antibodies.

A process control is just that, a control for the stain process. While the concept can be extended to the primary epitope also, this may not be practical in all cases. Purified protein epitopes are very expensive, and adding a dilution series of primary epitope to a control slide would create a unique and possibly... 

Our use of the process control slide during its development has already surprised us by demonstrating significant variations in lot-to-lot commercial detection systems. While we have yet to perform a systematic evaluation of... 

Proceed with the TUNEL Assay for Adherent Cells (Subheading 3.5.2.). Process all positive control slides in separate coplin jars to avoid introducing DNasel contamination into experimental samples. 

Control slides of known reactivity should be run with each set of slides immunostained. Appropriate controls should include an irrelevant antibody of the same immunoglobulin class at an equivalent concentration. 

Mark control slides and keep them in Coplin jar with 100 mM Tris-HCl, pH 8.0 until the PCR step. 

Cover each control slide with an appropriate reaction mix and seal with rubber cement and a coverslip. 

When control slides show optimum signal, but there is poor or no signal in the experimental slides, this may be because of ... 

Hapten-labeled DNA probes for FISH assays can be used for BISH assays and those probes can be purchased from various vendors. If commercial hapten-labeled DNA probes are not available, DNA probes can be labeled with haptens by nick translation or by PCR in a laboratory. Nick translation kit can be utilized for labeling DNAprobes with hapten (10976776001, Roche Applied Science, Germany). New probes must be analyzed for the specificity by FISH or BISH assays using a comparative genomic hybridization metaphase control slide. 

PBS). The drops are confined to areas where the sections are encompassed by the wax circles. After the slides have been treated horizontally for 5 min at room temperature, the slide is immersed in PBS in a Coplin jar to remove the SDS. The control slide not exposed to SDS is washed in a separate jar to avoid any contact with SDS. The slides are thoroughly washed three times for 5 min each with PBS, completely removing the SDS otherwise, residual SDS will denature the antibodies subsequently applied to the sections. 

Specimen tissue and negative reagent control slides show background staining. Positive and negative control tissue show appropriate specific staining. May involve several tissue elements such as connective tissue, adipose tissue and epithelium. 

Negative reagent control slide shows background. Positive control tissue, negative control tissue and specimen tissue show expected specific staining. 

Adipose or connective tissue in specimen, negative control tissue, positive control tissue and negative reagent control slides. Background in... 

Add phages to the chambers and incubate at 37°C for 1 h. As negative control for internalization, incubate a control slide with phages at 4°C for 1 h followed by two washes in ice cold PBS. Then, proceed to step 7 below. 

Assay Control The assay control involves omission of the primary antibody (test article or negative control antibody) during the staining reaction and permits determination of staining by the secondary or tertiary antibodies or other components of the reaction process. This control slide may or may not be included based on the immunohistochemical method chosen. 

Flow-control slide automatically actuated by height-controller paddle... 

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