Microalgae analysis

Fig. 2.31. Chromatogram of crude lutein from the microalga Chlorella vulgaris by HPLC analysis, A = lutein. Conditions column reversed-phase C18 column (250 X 4.6 mm i.d., 5 pm) mobile phase methanol-dichloromethane-acetonitrile-water (67.5 22.5 9.5 0.5, v/v) flow rate l.Oml/min detection at 450 nm (a). Chromatogram of crude lutein from the microalga Chlorella vulgaris by preparative HSCCC separation, A = lutein. Conditions column multilayer coil of 1.6mm i.d. PTFE tube with a total capacity of 230ml rotary speed 800rpm solvent system ra-hexane-ethanol-water (4 3 1, v/v) mobile phase lower phase (ethanol-water) flow rate lml/min detection at 254 nm sample size 200 mg retention of the stationary phase 58 per cent (b). Reprinted with permission from H.-B. Li el al. [70].

Feinberg DA (1984) Technical and economic analysis of liquid fuel production from microalgae. DOE Report SERI/TP-231-2608. Solar Energy Research Institute, Golden, CO... 

Currin, C. A., S. Y. Newell, and H. W. Paerl. 1995. The role of standing dead Spartina alterniflora and benthic microalgae in salt marsh food webs Considerations based on multiple stable isotope analysis. Marine Ecology Progress Series 121 99-116. 

There are lots of research applications published regarding PLE and analysis of various compoimds in food and agricultural materials, for example pesticides in rapeseed (38), babyfood (39) and strawberries (40), lipids in com and oats (41) and in egg-containing foods (42), acrylamide in food samples (43), carotenoids in processed food (44), xanthones and flavanones in root bark (45), tocopherols in seeds and nuts (46), antioxidants in microalgae (47) and anthocyanins and total phenolics in dried red grape skin (48). More discussions... 

It is not possible, at present, to provide either a detailed resource base assessment (e.g., potentially available water, land, or nutrient resources), or a detailed cost analysis of aquatic plant production. Thus, this review presents general concepts of aquatic biomass farming exemplified by three systems — microalgae farming for lipid fuel and chemicals production, cattail cultivation for conversion to alcohol fuels, and growing water hyacinths for methane gas generation. Wastewater aquaculture applications are not covered in this review nor are the actual conversion processes by which aquatic biomass would be converted to fuels. 

Benemann, J.R., et a "A Systems Analysis of Bioconversion with Microalgae", "Symposium Papers", Clean Fuels from Biomass and Wastes, Symposium sponsored by the Institute of Gas Technology, Orlando, Fla., Jan. 25-28, 1977 Institute of Gas Technology Chicago. 1977 pp 101-26. 

Chen X, Goh QY, Tan W, Hossain I, Chen WN, Lau R Lumostatic strategy for microalgae cultivation utilizing image analysis and chlorophyll a content as design parameters, Bio-resour Technol 102(10) 6005-6012, 2011. 

Williams PJleB, Laurens LML Microalgae as biodiesel and biomass feedstocks review and analysis of the biochemistry, energetics and economics, Energy Environ Sci 3 554-590, 2010. 

Lang, I., Hodac, L., Friedl, T., Feussner, I, (2011). Fatty acid profiles and their distribution patterns in microalgae a comprehensive analysis of more than 2000 strains from the SAG culture collection. BMC Plant Biol, lliX), 124. 

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