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Metal flavin

Thus, during the last decade, the method of cryoradiolytic reduction has emerged as a new tool to investigate critical intermediates of redox systems. X-ray-induced radiation chemistry is also increasingly recognized both as a potential source of misinterpretations due to measurement-induced changes of the sample, and as a new, important tool in X-ray crystallography, where the irradiation of protein crystals may be used to deliberately alter the redox state of metals, flavins, disulfides, and other cofactors - The... [Pg.158]

The disodium salt of 7-nitroso-8-hydroxyquinoline-5-sulphonic acid (Na2L) reacts with Cu " to form CuL,H20. Cu(C10 )2 reacts with 10-methyl-isoalloxazine (L) and aqueous formic acid to form [CuL2](C104)2,4H20, the structure of which is shown as (214). The complex serves as a model compound for metal-flavin enzymes and controverts earlier suggestions that... [Pg.324]

M. J. Clarke, M. G. Dowling, A. R. Garafalo and T. F. Brennan, Structural and electronic effects resulting from metal-flavin ligation,/. Biol. Chem., 1980,255,3472-3481. [Pg.75]

Fatty Acyl CoA Oxidation. The two flavoproteins which have been isolated that oxidize fatty acyl CoA compounds differ in cofactors as well as in substrate specificity. The yellow enzsrme, acting on longer fatty acid derivatives, may contain iron in addition to FAD. The green enzyme, acting on 3- to 8-carbon chains, contains 2 atoms of copper per FAD. As in the case of other metal-flavins, the copper appears to be required only for transfer to cytochrome c. The implicit role ascribed to the metal is difficult to reconcile with the recent report of additional ffavoproteins with low metal content required to mediate between the fatty acyl CoA oxidases and either dyes or cytochrome c. These enzymes, called electron-transferring ffavoprotein or ETF, exhibit peaks near 375 and 437 m/i with a shoulder at 460 m/i. [Pg.180]

Electrochemistry of transition metal complexes with flavins, nucleosides and their constituent bases. M. J. Clarke, Rev. Inorg. Chem., 1980, 2, 27-51 (56). [Pg.63]

Flavin-containing Baeyer-Villiger monooxygenases (BVMOs) represent nature s equivalent of conventional peracids or de novo designed metal complexes... [Pg.243]

Flavoprotein enzymes contain flavin mononucleotide (FMN) or flavin adenine dinucleotide (FAD) as prosthetic groups. FMN and FAD are formed in the body from the vitamin riboflavin (Chapter 45). FMN and FAD are usually tighdy—but not covalendy—bound to their respecdve apoenzyme proteins. Metalloflavopro-teins contain one or more metals as essential cofactors. [Pg.86]

In the first family, the metal is coordinated by one molecule of the pterin cofactor, while in the second, it is coordinated to two pterin molecules (both in the guanine dinucleotide form, with the two dinucleotides extending from the active site in opposite directions). Some enzymes also contain FejSj clusters (one or more), which do not seem to be directly linked to the Mo centers. The molybdenum hydroxylases invariably possess redox-active sites in addition to the molybdenum center and are found with two basic types of polypeptide architecture. The enzymes metabolizing quinoline-related compounds, and derivatives of nicotinic acid form a separate groups, in which each of the redox active centers are found in separate subunits. Those enzymes possessing flavin subunits are organized as a2jS2A2, with a pair of 2Fe-2S centers in the (3 subunit, the flavin in the (3 subunit, and the molybdenum in the y subunit. [Pg.167]

We next focus on the use of fixed-site cofactors and coenzymes. We note that much of this coenzyme chemistry is now linked to very local two-electron chemistry (H, CH3", CH3CO-, -NH2,0 transfer) in enzymes. Additionally, one-electron changes of coenzymes, quinones, flavins and metal ions especially in membranes are used very much in very fast intermediates of twice the one-electron switches over considerable electron transfer distances. At certain points, the chains of catalysis revert to a two-electron reaction (see Figure 5.2), and the whole complex linkage of diffusion and carriers is part of energy transduction (see also proton transfer and Williams in Further Reading). There is a variety of additional coenzymes which are fixed and which we believe came later in evolution, and there are the very important metal ion cofactors which are separately considered below. [Pg.205]

A still more complicated reaction is the chemiluminescent oxidation of sodium hydrogen sulfide, cysteine, and gluthathione by oxygen in the presence of heavy metal catalysts, especially copper ions 60>. When copper is used in the form of the tetrammin complex Cu(NH3) +, the chemiluminescence is due to excited-singlet oxygen when the catalyst is copper flavin mononucleotide (Cu—FMN), additional emission occurs from excited flavin mononucleotide. From absorption spectroscopic measurements J. Stauff and F. Nimmerfall60> concluded that the first reaction step consists in the addition of oxygen to the copper complex ... [Pg.79]

The systems where this type of reaction is produced may be metal-, heme- or flavin-dependent. In flavin-dependent monooxygenases, a flavin-oxygen intermediate reacts with the substrate, producing water in a second step and requiring cofactors for regeneration of the flavin moiety. The non-heme-dependent oxygenases include the... [Pg.103]

Enzymes containing amino acid radicals are generally associated with transition metal ions—typically of iron, manganese, cobalt, or copper. In some instances, the metal is absent it is apparently replaced by redox-active organic cofactors such as S -adenosylmethionine or flavins. Functionally, their role is analogous to that of the metal ion in metalloproteins. [Pg.158]

Vames AW, Dodson RB, Wehry EL (1972) Interactions of transition-metal ions with photoexcited states of flavins. Fluorescence quenching studies. J Am Chem Soc 94 946-950... [Pg.98]

The addition of cofactors to antibodies is a sure means to confer a catalytic activity to them insofar as this cofactor is responsible for the activity. Indeed for many enzymes, the interaction with cofactors such as thiamins, flavins, pyridoxal phosphate, and ions or metal complexes is absolutely essential for the catalysis. It is thus a question there of building a new biocatalyst with two partners the cofactor responsible for the catalytic activity, and the antibody which binds not only the cofactor but also the substrate that it positions in a specific way one with respect to the other, and can possibly take part in the catalysis thanks to some of its amino acids. [Pg.342]

Three other proteins with similar domain structure as that of FprA were reported in other bacteria (WasserfaUen et al. 1995 Gomes et al. 1997, 2000). The recombinant CthFprA and CthHrb, overexpressed in E. coli, were purified and characterized. Both FprA and Hrb were found to be present as dimers. Metal/cofactor analysis of the purified proteins revealed the presence of 2 mol each of iron and flavin (FMN) per mole dimer of Hrb and 4 mol of iron and 2 mol FMN per mole dimer of FprA. The EPR spectra of the purified proteins indicated that iron is present in a di-iron center in FprA and as a Fe(Cys)4 cluster in Hrb. [Pg.197]

In contrast to the use of self-assembly reactions and metal ion coordination preferences to direct the construction of mixed cofactor systems, the use of SPPS or selective chemical ligation allows for the direct covalent attachment of cofactors for the construction of mixed cofactor systems within de novo design. Figure 11 shows the flavocy-tochrome maquette constructed by Dutton and co-workers (149) using a flavin moiety covalently attached to a unique cysteine residue inside a four helix bundle with bis-histidine binding sites for heme... [Pg.431]

The enzymes of this type that have been characterized contain some type of redox-active cofactor, such as a flavin (3), or a metal ion (heme iron, non-heme iron, or copper), or both (4-6). Our understanding of the mechanism of these enzymes is most advanced in the case of the heme-containing enzyme cytochrome P450. But in spite of the availability of a crystal structure of an enzyme-substrate complex (7) and extensive information about related reactions of low molecular weight synthetic analogues of cytochrome P450 (8), a detailed picture of the molecular events that are referred to as "dioxygen activation" continues to elude us. [Pg.105]


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See also in sourсe #XX -- [ Pg.316 ]




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