Measurement of concentrations

While a laser beam can be used for traditional absorption spectroscopy by measuring / and 7q, the strength of laser spectroscopy lies in more specialized experiments which often do not lend themselves to such measurements. Other techniques are connnonly used to detect the absorption of light from the laser beam. A coimnon one is to observe fluorescence excited by the laser. The total fluorescence produced is nonnally proportional to the amount of light absorbed. It can be used as a measurement of concentration to detect species present in extremely small amounts. Or a measurement of the fluorescence intensity as the laser frequency is scaimed can give an absorption spectrum. This may allow much higher resolution than is easily obtained with a traditional absorption spectrometer. In other experiments the fluorescence may be dispersed and its spectrum detennined with a traditional spectrometer. In suitable cases this could be the emission from a single electronic-vibrational-rotational level of a molecule and the experimenter can study how the spectrum varies with level. 

Sputtered Neutral Mass Spectrometry (SNMS) is the mass spectrometric analysis of sputtered atoms ejected from a solid surface by energetic ion bombardment. The sputtered atoms are ionized for mass spectrometric analysis by a mechanism separate from the sputtering atomization. As such, SNMS is complementary to Secondary Ion Mass Spectrometry (SIMS), which is the mass spectrometric analysis of sputtered ions, as distinct from sputtered atoms. The forte of SNMS analysis, compared to SIMS, is the accurate measurement of concentration depth profiles through chemically complex thin-film structures, including interfaces, with excellent depth resolution and to trace concentration levels. Genetically both SALI and GDMS are specific examples of SNMS. In this article we concentrate on post ionization only by electron impact. 

The measurements of concentration gradients at surfaces or in multilayer specimens by neutron reflectivity requires contrast in the reflectivity fiDr the neutrons. Under most circumstances this means that one of the components must be labeled. Normally this is done is by isotopic substitution of protons with deuterons. This means that reflectivity studies are usually performed on model systems that are designed to behave identically to systems of more practical interest. In a few cases, however (for organic compounds containing fluorine, for example) sufficient contrast is present without labeling. 

In Raman spectroscopy the intensity of scattered radiation depends not only on the polarizability and concentration of the analyte molecules, but also on the optical properties of the sample and the adjustment of the instrument. Absolute Raman intensities are not, therefore, inherently a very accurate measure of concentration. These intensities are, of course, useful for quantification under well-defined experimental conditions and for well characterized samples otherwise relative intensities should be used instead. Raman bands of the major component, the solvent, or another component of known concentration can be used as internal standards. For isotropic phases, intensity ratios of Raman bands of the analyte and the reference compound depend linearly on the concentration ratio over a wide concentration range and are, therefore, very well-suited for quantification. Changes of temperature and the refractive index of the sample can, however, influence Raman intensities, and the band positions can be shifted by different solvation at higher concentrations or... 

Simulation of transport and measurement of concentration distribution of air pollutants... 

Therapeutic diug monitoring by measurement of concentrations at a defined time related to drug dosing. 

Gal-Or and Hoelscher (G5) have recently developed a fast and simple transient-response method for the measurement of concentration and volumetric mass-transfer coefficients in gas-liquid dispersions. The method involves the use of a transient response to a step change in the composition of the feed gas. The resulting change in the composition of the liquid phase of the dispersion is measured by means of a Clark electrode, which permits the rapid and accurate analysis of oxygen or carbon dioxide concentrations in a gas, in blood, or in any liquid mixture. 

Chemical kinetics is the study of the rates of chemical reactions. Its practice entails the measurement of concentrations as a function of time. These measurements are extended to other variables, such as the concentrations of additional species, pH, temperature, pressure, isotopic substitution, solvent, salt concentration, and so on. 

Two measures of concentration that are useful for the study of colligative properties, because they indicate the relative numbers of solute and solvent molecules, are mole fraction and molality. We first met the mole fraction, x, in Section 4.8, where we saw that it is the ratio of the number of moles of a species to the total number of moles of all the species present in a mixture. The molality of a solute is the amount of solute species (in moles) in a solution divided by the mass of the solvent (in kilograms) ... 

We use a different measure of concentration when writing expressions for the equilibrium constants of reactions that involve species other than gases. Thus, for a species J that forms an ideal solution in a liquid solvent, the partial pressure in the expression for K is replaced by the molarity fjl relative to the standard molarity c° = 1 mol-L 1. Although K should be written in terms of the dimensionless ratio UJ/c°, it is common practice to write K in terms of [J] alone and to interpret each [JJ as the molarity with the units struck out. It has been found empirically, and is justified by thermodynamics, that pure liquids or solids should not appear in K. So, even though CaC03(s) and CaO(s) occur in the equilibrium... 

An important application of the Nernst equation is the measurement of concentration. In a concentration cell, the two electrodes are identical except for their concentrations. For such a cell, E° = 0 and at 25°C the potential corresponding to the cell reaction is related to Q by E = —(0.025693 V//z) In Q. For example, a concentration cell having two Ag+/Ag electrodes is... 

Obviously, the fast-fill-and-hold method is preferred from the viewpoint of elapsed time. More importantly, the fed-batch method requires an accurate process model that may not be available. The fast-fill-and-hold method can use a process model or it can use a real-time measurement of concentration. 

J.B. Moss 1980, Simultaneous measurements of concentration and velocity in an open premixed turbulent flame. Combust. Sci. Tech. 22 119-129. 

Any solution contains at least two chemical species, the solvent and one or more solutes. The mass of a solution is the sum of the masses of the solvent and all dissolved solutes. To answer questions such as How much is there about solutions, we need to know the amount of each solute present in a specified volume of solution. The amount of a solute in a solution is given by the concentration, which is the ratio of the amount of solute to the amount of solution. In chemistry the most common measure of concentration is molarity (M). Molarity is the number of moles of solute (n) divided by the total volume of the solution (V) in liters ... 

Spectrophotometric and spectrofluorimetric methods provide a wealth of information concerning structural determinations (identification, purity and precise measurement of concentration) and chemical changes in alkaloids. These techniques yield both quantitative and qualitative data on the effect of solvents, pH and other physiological conditions [141-143]. X-ray crystallography, H and NMR spectroscopy, infrared spectroscopy (IR) and circular dichroic spectroscopy were also used to study the physical properties... 

Measurement of concentration profile of oxygen in the lower thermosphere of the Earth with the help of semiconductor sensors... 

Concentration assays are often the least demanding, since usually the component to be measured is abundant and minor components scarce. Even if resolution is poor or there is detector noise, accurate measurements of concentration can still be obtained. In concentration assays, the principal requirements are stringency in the precision of sample dilution and measurement of column losses of the major component. Detector calibration, another important issue in concentration assays, has been discussed above. 

The limitation of Eq. (2.1) is that measurement of concentrations of solute within different parts of the membrane is very inconvenient. However, since we can estimate (or possibly measure) the distribution coefficients between bulk water and the membrane, log Kj (the pH-dependent apparent partition coefficient), we can convert Eq. (2.1) into a more accessible form... 

Perhaps the most useful measure of concentration is molarity. Molarity is defined as the number of moles of solute per liter of solution ... 

Most of the factors that affect the rate of a reaction are qualitative or semiquantitative, but the dependency of the rate on concentration (or pressure, which is a measure of concentration) may be... 

Volkl K.P., Opitz N., Lubbers D.W., Continuous measurement of concentrations of alcohol using a fluorescence-photometric enzymic method, Fresenius J. Anal. Chem. 1980 301 162. 

Quantitative and qualitative analytical techniques are of considerable importance in various aspects of life sciences for the purpose of detection, identification and measurement of concentrations of biologically important molecules, cells, chemical compounds, etc. 

Measurement of concentrations of naturally occurring radionuclides and natural background doses in the environment as a baseline for studies on radiation effects (Templeton et al. 1971)... 

Single-ion electrodes for measurement of concentration of individual species. 

P is total pressure, and only NH3 is present initially. Justify your answer quantitatively, for example, by using the experimental data in conjunction with the form given in (a). Use partial pressure as a measure of concentration. 

The effects of constant and proportional errors on a measurement of concentration. 

Measurement of Concentration-Time Data and Possible Problems... 

Introduction 257 The Basics of Michaelis-Menten Kinetics 259 Hydrogenation From a Kinetic Viewpoint 263 Measurement of Concentration-Time Data and Possible Problems 263... 

The concept of mole fraction of a component used in Equation (4.1) is a convenient measure of concentration when dealing with trace quantities and dilute solutions, often experienced in environmental systems. This is especially the case with transport phenomena and equilibrium between phases, where it results in simple quantitative expressions. The phenomena of interest when dealing with the exchange of odorous compounds and oxygen between wastewater and a sewer atmosphere are, in this respect, relevant examples. 

Chemical sensors are becoming more and more important in any area where the measurement of concentrations of volatile compounds is relevant for both control and analytical purposes. They have also found many applications in sensor systems called electronic noses and tongues. 

In most natural situations, physical and chemical parameters are not defined by a unique deterministic value. Due to our limited comprehension of the natural processes and imperfect analytical procedures (notwithstanding the interaction of the measurement itself with the process investigated), measurements of concentrations, isotopic ratios and other geochemical parameters must be considered as samples taken from an infinite reservoir or population of attainable values. Defining random variables in a rigorous way would require a rather lengthy development of probability spaces and the measure theory which is beyond the scope of this book. For that purpose, the reader is referred to any of the many excellent standard textbooks on probability and statistics (e.g., Hamilton, 1964 Hoel et al., 1971 Lloyd, 1980 Papoulis, 1984 Dudewicz and Mishra, 1988). For most practical purposes, the statistical analysis of geochemical parameters will be restricted to the field of continuous random variables. 

In manufacturing and processing in GRP plant, styrene and solvents must be extracted to prevent excessive concentrations in the air. In 1987 the permitted work-place concentration for styrene was reduced from lOOppm to 20ppm. From 31-12-94 values above 20ppm are no longer permitted where UP resins are processed manually. Exact measurement of concentrations is essential and suitable extraction systems installed. 

The kinetic investigation of a reaction is carried out to establish the rate law and to measure the rate coefficients. The first step is to identify or examine the role of each component, i.e. to determine the order of reaction with respect to each reactant or product. There are various methods, which can be used to determine the order of reaction with respect to a reactant. However, every method requires essentially the measurement of concentration of the reactant or product at various time intervals. 

Here, r is a reaction rate, CA and CB are measures of concentration, and the remaining terms represent parameters to be estimated. 

Experimental measurements of concentration profiles within the bed have been made using a pressure transducer attached to a probe whose vertical position in the bed could be varied. The voidage e of the bed at a given height may then be calculated from the local value of the pressure gradient using equation 6.1, from which ... 

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