Major outer membrane proteins

Kersten, G. F. A., Van de Put, A., Teerlink, T., Beuvery, E. C., and Crommelin, D. J. A. (1988a). Immunogenicity of liposomes and iscoms containing the major outer membrane protein of Neisseria gonorrhoeae -Influence of protein content and liposomal bilayer composition. Inf. Immun., 56. 1661-1664. 

Alam, M., Miyoshi, S.-L, Tomochika, K.-L, and Shinoda, S. (1996). Purification and characterization of novel hemagglutinins from Vibrio mimicus A 39 kilodalton major outer membrane protein and lipooligosaccharide. Infect. Immun. 64,4035-4041. 

Bodilis, J. and Barray, S. 2006. Molecular evolution of the major outer-membrane protein gene (oprF) of Pseudomonas. Microbiology-SGM, 152 1075-1088. 

Hitchcock, PJ. Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dode-cyl sulfate-polyacrylamide gel electrophoresis stable association of lipopolysaccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae. Infect Immun 46 (1984) 202-212. 

Chromosomal mutants of Ps. aeruginosa produced by stepwise exposure to the antibiotic polymyxin are resistant to this drug and to EDTA [175]. The mechanism of this resistance is related to a defective self-promoted uptake pathway in which the cells contain increased amounts of a major outer membrane protein (HI) with a corresponding decrease in envelope Mg2+ [175, 176]. Protein HI may replace Mg2+ at cross-bridging sites with LPS these sites are normally those at which interaction occurs with Mg2+, such as EDTA, or at which displacement of Mg2+ takes place, for example, polymyxin [177, 178],... 

Berberine sulfate was found to have no effect on the growth of uropathogenic E. coli or on the synthesis of major outer membrane proteins, but it did block adhesion of the organisms to erythrocytes and to epithelial cells. The decreased adhesion was accompanied by a reduction in the synthesis of fimbria subunits and in the expression of assembled fimbriae, suggesting that the anti-infective activity of the alkaloid in . co/i-induced UTI may be mediated by the selective suppression of the synthesis and assembly of fimbriae by these organisms [207]. 

Edmonds, M D., Cloeckaert, A., Elzer, P.H. 2002. Brucella species lacking the major outer membrane protein Omp25 are attenuated in mice and protect against Brucella melitensis and Brucella ovis. 88 205-221. 

Lutkenhaus JF (1977) Role of a major outer membrane protein in Escherichia coli. J Bacteriol 131 631-637. 

Sonntag, I., Schwartz, H., Hirota, Y. and Henning, U., 1978, Cell envelope and shape of Escherichia coli multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins. J. Bacteriol. 136 280-285. 

Figure 3.27 SDS-polyacrylamide Gel Electrophoresis Patterns of the Major Outer Membrane Proteins of Four Gram-negative Species. The isolated membrane fractions have been heated to 100X in SDS before electrophoresis...

Table 3.2 Properties of the Constitutive Major Outer Membrane Proteins of Escherichia coli K12...

The largest major outer membrane protein, designated protein a, is present in appreciable quantity in E. coli K12 only when the organism is grown at 37°C or above. Protein a is able to act as a protease catalysing the in vivo cleavage of the outer membrane receptor for ferric enterobactin (p. 94). An additional role for protein a in the regulation of capsular polysaccharide formation has also been proposed. 

Only limited enzymic activity is associated with the outer membrane. A major outer membrane protein of enteric bacteria, protein a, is endowed... 

Data computed for the major outer membrane protein of a Pseudomonas fluorescens strain used for the calculation of the molecular composition of AzospiriUum brasilense ... 

A study performed by Saurabh Dixit et al. paves the way for the development of a vaccination strategy against Chlamydia trachomatis, the most frequently reported sexually transmitted bacterial infection worldwide. It spotlights the potential of a PLA-PEG-based nanoparticle formulation containing a major outer membrane protein of Chlamydia trachomatis in inducing a sustained enhanced immime response [57]. 

Since the first 370-fold purification of RNase Tl (1), a number of important improvements have been made to increase the yield, purity, and efficacy of purification (31). The improvements in separation techniques include various affinity chromatographies that rely on such adsorbents as 2, 3 -cGMP, aminophenylphos-phoryl-GMP (50), 5 -GMP (40), and 2, 5 -GpG (51). In addition, the RNase Tl gene cloned into an expression—secretion vector efficiently produces the enzyme from . coli hosts (yield of 20 mg enzyme per liter of culture) (49). In this system, the RNase Tl gene is expressed under the inducible control of the lac promoter-operator as a fusion protein with the signal peptide of OmpA, the major outer membrane protein of . coli. The in vivo cleavage of the fusion... 

Because of the limited number of functions of the outer membrane, it consists of much fewer protein components than does the cytoplasmic membrane.As will be discussed later, the major outer membrane proteins exist in extremely large quantities, of the order of 1-8 x 10 molecules/cell, suggesting that they occupy a considerable part of the outer membrane structure. This unique feature of the outer membrane provides us with an excellent system for the investigation of the mechanism of biosynthesis and the mode of assembly of individual outer membrane proteins, their interaction with other proteins, phospholipids, and lipopolysaccharide, and their functions. 

These studies indicate that the major outer membrane proteins (and possibly some cytoplasmic membrane proteins) are produced in a somewhat different manner than the cytoplasmic proteins. Furthermore, it appears that individual outer membrane proteins have their own specific biosynthetic systems. It has been suggested that such specific systems for the outer membrane protein biosynthesis may be achieved by one mechanism or a combination of the following mechanisms (a) differentiation of ribosomes, (b) compartmentalization of ribosomes, and (c) differentiation of factors required for protein synthesis. ... 

As we have already seen, the peak 11 protein is one of the four major outer membrane proteins. This protein is a very peculiar lipoprotein of... 

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